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植物研究 ›› 2006, Vol. 26 ›› Issue (2): 233-237.doi: 10.7525/j.issn.1673-5102.2006.02.025

• 论文 • 上一篇    下一篇

植物叶际微生物提取方法研究

周 育1,2;乔雄梧1;王 静1;崔中利2;李顺鹏2   

  1. 1.山西省农科院山西省农药重点实验室,太原 030031 2.南京农业大学农业部农业环境微生物工程重点开放实验室,南京 210095
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-03-20 发布日期:2006-03-20
  • 通讯作者: 周 育
  • 基金资助:
     

Extraction methods of microorganisms from phyllosphere

ZHOU Yu;QIAO Xiong-Wu;WANG Jing;CUI Zhong-Li;LI Shun-Peng   

  1. 1.Shanxi Key Laboratory of Pesticide Science, Shanxi Academy of Agricultural Sciences, Taiyuan 030031 2.College of Life science, Nanjing Agriculture University, Key Lab of Microbiological Engineering of Aricultural Environment, Ministry of Agriculture,Nanjing 210095
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-03-20 Published:2006-03-20
  • Contact: ZHOU Yu
  • Supported by:
     

摘要: 实验以菜豆叶子为材料,定量比较了不同叶际微生物提取方法的提取效果,为叶际微生物的研究提供有效的手段。通过实验研究得出,叶际微生物用不同的提取方法和提取溶液提取效果有很大的差别。用超声波提取,随着超声时间的延长,微生物数量先会有一个增长的过程达到最佳效果后又会减少,最佳提取时间为4 min左右;水和磷酸缓冲液两种提取溶液的提取效果没有显著的差别;匀浆的提取方法与超声波提取法相比,提取量有明显增多甚至多出一倍左右,前者最大提取量为1.12×107个/g鲜叶;而后者为7.67×106个/g鲜叶;叶子的采样量一般是在4~5 g为宜。通过实验还得出随着叶龄的增大叶际微生物数量会显著的增多,但对于叶际微生物的研究还要综合考虑其他各种因素,随具体的目的才能确定最适合的叶龄。

关键词: 叶际, 微生物, 提取方法

Abstract: The extraction methods of microorganisms from the bean’s leaves were investigated and the efficiency of different methods was compared. Results showed that different extraction methods and solvents led to quite different results. By ultrasonic cleaner method, the number of microorganisms increased to a maximum with the time prolonging and then decrease, the most effective treatment time is about four minates. There are no significant difference between sterile water and phosphate buffer. Comparing with ultrasonic cleaner method, extraction efficiency of homogenate method was much higher. The maximum yield of the former was about 7.67×106 cfu/g fresh leaves while the latter was up to 1.12×107 cfu/g. The optimal weight of the leaves sample was four to five g. Leaf age influenced the abundance of microbial population significantly, but the leaf age of a sample for a specific purpose still depends on the other factors.

Key words: phyllosphere, microorganism, extraction method

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