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Bulletin of Botanical Research ›› 2009, Vol. 29 ›› Issue (3): 352-356.doi: 10.7525/j.issn.1673-5102.2009.03.019

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Establishment and Optimization of SRAP-PCR Reaction System in Banana Genome

WEI Jun-Ya;LIU De-Bing;WEI Shou-Xing;XIE Zi-Si;CHEN Ye-Yuan*   

  1. (1.Germplasm Research Institute of Tropical Crops of Chinese Academy of Tropical Agriculture Sciences,Key Tropical Crops Germplasm Utilization,Ministry of Agriculture P.R. of China,Danzhou571737) (2.College of Horticulture and Forestry,Hainan University,Danzhou571737)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-05-20 Published:2009-05-20
  • Contact: CHEN Ye-Yuan
  • Supported by:

Abstract: In order to establish and optimize the SRAP molecular marker system in Musa spp.,the concentrations of Mg2+, dNTPs, Taq DNA polymerase, primers which affect the SRAP-PCR reactions were optimized. The optimum system was as follows: Mg2+ 2.5 mmol·mL-1, dNTPs 250 μmol·L-1, Taq DNA polymerase 1.0 U, primer 0.5 μmol·L-1, template DNA 20 ng, 10×PCR buffer 2.5 μL.The total volume of reaction was 25 μL. Amplications were carriyed out on 29 banana cultivars genome using this optimum system. The results showed that the system was steady and reliable and would be helpful to study origin and evolution of Musa spp..

Key words: Musa spp., SRAP, Optimization

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