Abstract: In order to establish and optimize the SRAP molecular marker system in Musa spp.，the concentrations of Mg²⁺, dNTPs, Taq DNA polymerase, primers which affect the SRAP-PCR reactions were optimized. The optimum system was as follows: Mg²⁺ 2.5 mmol·mL^-1, dNTPs 250 μmol·L^-1, Taq DNA polymerase 1.0 U, primer 0.5 μmol·L^-1, template DNA 20 ng, 10×PCR buffer 2.5 μL.The total volume of reaction was 25 μL. Amplications were carriyed out on 29 banana cultivars genome using this optimum system. The results showed that the system was steady and reliable and would be helpful to study origin and evolution of Musa spp..

Key words: Musa spp., SRAP, Optimization