Abstract: Removal effect of two species of filamentous green algae, Cladophora fracta and Spirogyra longata, on phenol at low concentration was studied. The results show that the removal rate of C.fracta and S.longata towards phenol are 86% and 75.63% under absence of between-subjects effects, respectively. It was indicated the temperature is the primary factor to C.fracta and the exposure time is the primary factor to S.longata. The removal rate of C.fracta and S.longata towards phenol were 88.98% and 75.63% under presence of between-subjects effects, respectively. It was indicated the temperature is the primary factor to C.fracta and the exposure time is the primary factor to S.longata. The best combination for removing phenol by C.fracta is temperature 18℃, exposure time 2 h and algal weight 0.5 g and the best combination for removing phenol by S.longata is temperature 8℃, exposure time 1 h and algal weight 0.5 g.

Key words: phenol, filamentous green algae, Cladophora fracta, Spirogyra longata