Abstract: In this study, by using the leaves of seedling stage (tillering stage), shooting stage, booting stage and anthers of PMC meiotic stage, microspore 2-3 nuclei stage, pollen grain as experimental materials, proteome approaches were applied to a comparative analysis of heterosis in wheat F1-hybrid which was bred through hybridization of CMS with restoring line. The results indicated that three types of protein expression levels, super-parents type, parent di-type, low-parents type, were observed among F1-hybrid and parents. The frequence of protein expression was showed as parent di-type>low-parents type>super-parents type. A total of 80 protein spots were detected in F1-hybrid and parents, 17 protein spots have been identified by MALDI-YOF MS. These protein spots were involved in diverse biological processes, including DNA and protein synthesis, energy metabolism, stress response, gene transposition and photosynthesis. In the F1-hybrid, at seedling stage, leaf aerial traits such as leaf fresh weight (LFW), leaf dry weight (LDW) and leaf number, show a tendency to its parental lines when compared to parent, these results were consistent to proteome analysis results which showed most of protein spots expression in F1-hybrid exhibiting parent di-type. However, LFW, LDW, and total leaf number exhibited obviously heterosis because of tillering number (TN) increasing in F1-hybrid, it is necessary to further study the relationship between the heterosis and proteome change.

Key words: proteomic, parent di-type, heterosis, MS, wheat