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Bulletin of Botanical Research ›› 2023, Vol. 43 ›› Issue (5): 756-767.doi: 10.7525/j.issn.1673-5102.2023.05.012

• Molecular biology • Previous Articles     Next Articles

Cloning and Expression Analysis of Catalase Gene in Lilium pumilum

Yu SONG1,2, Wenhao LIN1,3, Yibo JING1,2, Yi DONG1,2, Shumei JIN1()   

  1. 1.Key Laboratory of Saline-alkai Vegetation Ecology Restoration,Ministry of Education,College of Life Sciences,Northeast Forestry University,Harbin 150040
    2.Aulin College,Northeast Forestry University,Harbin 150040
    3.College of Forestry,Northeast Forestry University,Harbin 150040
  • Received:2023-07-12 Online:2023-09-20 Published:2023-09-05
  • Contact: Shumei JIN E-mail:jinshumei1972@163.com
  • About author:SONG Yu(2001—),male,undergraduate student,mainly engaged in the research on the resistance of Lilium pumilum.
  • Supported by:
    Undergraduate Training Programs for Innovations by NEFU(180602011)


To explore the relationship between the CatalaseCAT) gene and saline-alkali stress tolerance of Lilium pumilum, the CAT gene was successfully cloned from L. pumilum bulb. The length of region ORF was 1 479 bp, encoding 492 amino acids, and sequence alignment and phylogenetic tree analysis were performed, and the Catalase protein of L. pumilum was found to be closely related to the Catalase proteins of L. sargentiaeAnanas comosusElaeis guineensis and other plants. The Catalase protein was induced to express, and purified in vitro with 1 mol·L-1 IPTG as the inducer after constructing prokaryotic expression vector pQE-LpCat. The results showed that the growth concentration of bacteria solution containing PQE-LpCat protein was higher than that of the control strain under the stress of 50 mmol·L-1 NaHCO3. Under the stress of 1 mol·L-1 NaHCO3 and 2.5 mol·L-1 H2O2, less wilting was observed in plants overexpressing the LpCAT gene compared to wild-type. Determination of the physiological indexes including net photosynthetic rate, stomatal conductance, intercellular CO2 and transpiration rate, H2O2 concentration and contents of malondialdehyde(MDA) showed that tobacco plants overexpressing the LpCat gene were more tolerant to saline and alkali stress than wild-type tobacco plants.

Key words: Lilium pumilum, Catalase gene, saline-alkaline stress

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