Pollen Morphological Characters of 20 Species from Camellia Sect. Theopsis and Sect. Eriandria

doi:10.7525/j.issn.1673-5102.2023.03.017

Abstract

Abstract:

To provide references for the phylogenetic evolution， classification and identification of plants in Camellia Sect. Theopsis and Camellia Sect. Eriandria were studied by pollen morphology. 20 species of the two sections， pollen shape and exine sculpture were used as materials and observed by scanning electron microscopy（SEM）. Results indicated：（1）Pollen size of 20 species mainly belonged to medium（25-50 μm） and large（50-100 μm） pollen. The pollens were all single-grain pollen with three grooves， three-cracked in the polar view and long spherical in the equatorial view. （2）There were three types of pollen exine sculpture， including foveolate， granulate and verrucate. C. elongata was foveolate type， C. forrestii， and C. forrestii var. acutisepala were verrucate type， and the other 17 species were granulate type. We considered that the evolution trend of pollen exine sculpture of the two sections was foveolate→granulate→verrucate. The results of pollen exine sculpture types supported the view that Sect. Theopsis and Sect. Eriandria were a monophyletic clade， while the taxonomic status of C. tsofuii and C. lipoensis should be further studied.

Key words: ornamental plants, Camellia Sect.Theopsis, Camellia Sect.Eriandria, pollen

CLC Number:

S685.14

Yali ZHANG, Naiying YANG, Yao SONG, Xiangpeng LI, Weizhen GUO, Youming CAI. Pollen Morphological Characters of 20 Species from Camellia Sect. Theopsis and Sect. Eriandria[J]. Bulletin of Botanical Research, 2023, 43(3): 470-480.

Figures/Tables 4

Table 1

Information of 20 species of Sect. Theopsis and Sect. Eriandria

组

Sect.

编号

No.

种名

Species

采集地

Collection Site

凭证标本

Voucher

连蕊茶组

Camellia Sect. Theopsis

尖连蕊茶

C.cuspidata

浙江省&安徽省，徽杭古道

Huihang Gudao，Zhejiang & Anhui

CSH0187864

浙江尖连蕊茶

C.cuspidata var. chekiangensis

浙江省，苍南县

Cangnan Xian，Zhejiang

CSH179224

长管连蕊茶

C.elongata

四川省，峨眉山

Emei Shan，Sichuan

CSH179204

柃叶连蕊茶

C.euryoides

湖南省，桂东县

Guidong Xian，Hunan

CSH0179221

蒙自连蕊茶

C.forrestii

云南省，薄竹山

Bozhu Shan，Yunnan

CSH179246

尖萼蒙自连蕊茶

C.forrestii var. acutisepala

云南省，薄竹山

Bozhu Shan，Yunnan

CSH179245

毛花连蕊茶

C.fraterna

浙江省，苍南县

Cangnan Xian，Zhejiang

CSH0179225

荔波连蕊茶

C.lipoensis

贵州省，荔波县

Libo Xian，Guizhou

CSH0179294

川鄂连蕊茶

C.rosthorniana

四川省，峨眉山

Emei Shan，Sichuan

CSH179212

毛枝连蕊茶

C.trichoclada

浙江省，苍南县

Cangnan Xian，Zhejiang

CSH179223

金屏连蕊茶

C.tsingpienensis

云南省，马关县

Maguan Xian，Yunnan

CSH0186790

毛萼金屏连蕊茶

C.tsingpienensis var. glabrisepal

广西省，大明山

Daming Shan，Guangxi

CSH0179257

作孚连蕊茶

C.tsofuii

重庆市，缙云山

Jinyun Shan，Chongqing

CSH0187824

毛蕊茶组

Camellia Sect. Eriandria

心叶毛蕊茶

C.cordifolia

广东省，天井山

Tianjing Shan，Gaungdong

CSH0179289

光萼心叶毛蕊茶

C. cordifolia var. glabrisepala

广东省，天井山

Tianjing Shan，Gaungdong

CSH0179283

四川毛蕊茶

C.lawii

重庆市，缙云山

Jinyun Shan，Chongqing

CSH0187828

广东毛蕊茶

C.melliana

广东省，南昆山

Nankun Shan，Gaungdong

CSH179278

斑枝毛蕊茶

C.punctata

四川省，乌尤寺

Wuyou Si，Sichuan

CSH0179209

柳叶毛蕊茶

C.salicifolia

广东省，中山大学

Sun Yat-sen University,Guangdong

CSH0187841

小果毛蕊茶

C.villicarpa

四川省，峨眉山

Emei Shan，Sichuan

CSH0179212

Table 1

Table 2

Pollen morphological characters of 20 species of Sect. Theopsis and Sect. Eriandria

编号 No.	种名 Species	花粉大小 Pollen size /μm	极轴长（P） Polar axis /μm	赤道轴长（E） Equatorial axis/μm	极轴长/赤道轴长 P/E
1	尖连蕊茶 C.cuspidata	（51.75~63.96）×（27.99~37.49）	58.26±3.78	31.74±2.05	1.84±0.16
2	浙江尖连蕊茶 C.cuspidata var. chekiangensis	（52.74~66.30）×（30.79~37.23）	60.73±3.37	34.21±1.69	1.78±0.11
3	长管连蕊茶 C.elongata	（38.81~48.10）×（26.59~33.35）	44.16±1.72	30.01±1.33	1.47±0.08
4	柃叶连蕊茶 C.euryoides	（49.48~60.56）×（26.39~32.96）	55.62±2.70	32.96±1.46	1.90±0.14
5	蒙自连蕊茶 C.forrestii	（48.72~62.22）×（23.77~36.04）	56.13±3.44	30.76±2.45	1.84±0.17
6	尖萼蒙自连蕊茶 C.forrestii var. acutisepala	（43.61~60.16）×（23.63~33.90）	49.82±3.53	29.17±1.71	1.71±0.15
7	毛花连蕊茶 C.fraterna	（52.71~71.20）×（34.37~43.52）	61.46±4.61	38.89±2.54	1.59±0.18
8	荔波连蕊茶 C.lipoensis	（49.08~64.89）×（29.82~38.61）	57.68±4.32	33.71±2.02	1.72±0.17
9	川鄂连蕊茶 C.rosthorniana	（43.56~52.28）×（25.54~32.07）	48.73±1.89	28.11±1.40	1.74±0.10
10	毛枝连蕊茶 C.trichoclada	（46.70~64.95）×（24.93~39.42）	58.44±4.25	33.24±2.59	1.77±0.19
11	金屏连蕊茶 C.tsingpienensis	（38.91~52.20）×（25.32~33.59）	47.43±2.67	28.89±1.99	1.65±0.15
12	毛萼金屏连蕊茶 C.tsingpienensis var. pubisepala	（49.62~62.15）×（29.30~38.53）	57.53±3.03	32.81±1.82	1.76±0.15
13	作孚连蕊茶 C.tsofuii	（50.28~62.42）×（28.04~33.59）	56.49±2.74	30.33±1.11	1.86±0.10
14	心叶毛蕊茶 C.cordifolia	（38.27~48.95）×（24.17~29.15）	45.17±2.29	27.09±1.18	1.67±0.10
15	光萼心叶毛蕊茶 C.cordifolia var. glabrisepala	（40.11~52.93）×（24.70~30.79）	47.06±2.75	27.58±1.69	1.71±0.16
16	四川毛蕊茶 C.lawii	（47.41~67.26）×（25.03~40.84）	58.79±5.21	32.00±3.36	1.85±0.21
17	广东毛蕊茶 C.melliana	（46.39~57.66）×（27.20~36.34）	51.44±2.60	31.21±1.77	1.65±0.12
18	斑枝毛蕊茶 C.punctata	（41.54~52.98）×（24.62~31.95）	45.54±2.64	27.59±1.92	1.61±0.14
19	柳叶毛蕊茶 C.salicifolia	（42.79~53.61）×（24.08~33.37）	48.98±2.22	29.95±2.00	1.64±0.11
20	小果毛蕊茶 C.villicarpa	（41.11~55.49）×（24.57~32.37）	51.33±2.77	27.68±1.85	1.86±0.17

Table 2

Fig.1

References 25

1	倪穗，李纪元.我国连蕊茶组植物资源及其园林应用前景［J］.浙江林业科技，2005，25（5）：70-73.
	NI S， LI J Y.Resources of Sect.Theopsis of Camellia and its ornamental prospect in China［J］.Journal of Zhejiang Forestry Science and Technology，2005，25（5）：70-73.
2	张亚利，郭卫珍，李湘鹏，等.“微”观茶花：束花茶花发展简纪［M］.北京：中国建筑工业出版社，2018：26-43，88-175.
	ZHANG Y L， GUO W Z， LI X P，et al.‘Micro’ observing on Camellias：a brief introduction to the development of cluster-flowering camellias［M］.Beijing：China architecture and building press，2018：26-43，88-175.
3	张亚利，杜诚，胡永红.山茶属连蕊茶组和毛蕊茶组资源及其种质创新研究进展［J］.亚热带植物科学，2021，50（4）：323-332.
	ZHANG Y L， DU C， HU Y H.Resources of Camellia Sect.Theopsis and Sect.Eriandria and germplasm innovation［J］.Subtropical Plant Science，2021，50（4）：323-332.
4	SEALY J R.A revision of the genus Camellia ［M］.London：The Royal Horticultural Society，1958：48-107.
5	CHANG H T， BRUCE B.Camellias［M］.Oregon：Timber Press，1984：157-207.
6	闵天禄.世界山茶属的研究［M］.昆明：云南科学技术出版社，2000：152-209.
	MIN T L.Monograph of the genus Camellia ［M］.Kunming：Yunnan Science and Technology Press，2000：152-209.
7	韦仲新，闵天禄， ZAVADA M S.山茶属的花粉形态及其分类学意义［J］.云南植物研究，1992，14（3）：275-282.
	WEI Z X， MING T L， ZAVADA M S.Pollen morphology of Camellia（THEACEAE） and its taxonomic significance［J］.Acta Botanica Yunnanica，1992，14（3）：275-282.
8	敖成齐，陈功锡，张宏达，等.山茶属短柱茶组花粉形态的研究［J］.华南农业大学学报，2001，22（2）：66-68.
	AO C Q， CHEN G X， ZHANG H D，et al.Study on the pollen morphology of Section Paracamellia in genus Camellia ［J］.Journal of South China Agricultural University，2001，22（2）：66-68.
9	倪穗，李纪元，田敏，等.红山茶组植物花粉外壁纹饰特征及其演化关系［J］.南京林业大学学报（自然科学版），2007，31（4）：16-20.
	NI S， LI J Y， TIAN M，et al.Pollen exine sculpture of Sect.Camellia in Genus Camellia and its taxonomic significance［J］.Journal of Nanjing Forestry University（Natural Sciences Edition），2007，31（4）：16-20.
10	谢一青.小果油茶农家品种花粉形态及亲缘关系分析［J］.南京林业大学学报（自然科学版），2016，40（3）：26-32.
	XIE Y Q.Analysis of pollen morphological difference and relationship of Camellia meiocarpa native varieties［J］.Journal of Nanjing Forestry University（Natural Sciences Edition），2016，40（3）：26-32.
11	石思云，吴雯霏，崔佳，等.10种金花茶组植物的花粉形态及分类学意义［J］.广西植物，2022，42（1）：68-77.
	SHI S Y， WU W F， CUI J，et al.Pollen morphology and taxonomic significance of ten species of Sect Chryantha ［J］.Guihaia，2022，42（1）：68-77.
12	薛晓明，周用武，方彦.4种山茶属植物花粉形态的扫描电子显微观察［J］.贵州农业科学，2012，40（5）：25-28.
	XUE X M， ZHOU Y W， FANG Y.Observation of pollen morphology of four species of Genus Camellia under scanning electron microscope［J］.Guizhou Agricultural Sciences，2012，40（5）：25-28.
13	许林，杜克兵，陈法志，等.川鄂连蕊茶花粉的形态、生活力及贮藏力研究［J］.园艺学报，2010，37（11）：1857-1862.
	XU L， DU K B， CHEN F Z，et al.Pollen morphology，vitality and storage capacity of Camellia rosthorniana Hand.-Mazz［J］.Acta Horticulturae Sinica，2010，37（11）：1857-1862.
14	李广清，孙立，刘燕.山茶属连蕊茶组6种植物花粉形态特征研究［J］.热带亚热带植物学报，2005，13（1）：40-44.
	LI G Q， SUN L， LIU Y.Pollen features of 6 species in Sect.Theopsis of genus Camellia ［J］.Journal of Tropical and Subtropical Botany，2005，13（1）：40-44.
15	敖成齐.山茶属Camellia植物花粉形态的光学显微镜观察［J］.安徽师范大学学报（自然科学版），2004，27（3）：318-321.
	AO C Q.Morphology of pollen grains in Camellia under light microscope［J］.Journal of Anhui Normal University（Natural Science），2004，27（3）：318-321.
16	敖成齐，陈功锡，张国萍，等.山茶属花粉外壁表面微形态特征的研究［J］.云南植物研究，2002，24（5）：619-626.
	AO C Q， CHEN G X， ZHANG G P，et al.Study on micromorphology of pollen exine surface in Camellia ［J］.Acta Botanica Yunnanica，2002，24（5）：619-626.
17	王开发，王宪曾.孢粉学概论［M］.北京：北京大学出版社，1983：21-34
	WANG K F， WANG X Z.An introduction to palynology［M］.Beijing：Peking University Press，1983：21-34.
18	海德玛莉·哈布里特，西尔维亚·乌尔里希，弗里盖尔·格里姆松，等.图解花粉术语［M］.2版.姚轶锋，李金峰，谢淦，等，译.武汉：湖北科学技术出版社，2021：8-52.
	HALBRITTER H， SILVIA U， FRIOGEIR G，et al.Illustrated pollen terminology［M］.2nd ed.YAO Y F，LI J F，XIE G，et al，trans.Wuhan：Hubei Science & Technology Press，2021：8-52.
19	黄华，赖华清，蒋勇，等.箭叶淫羊藿与近缘种天平山淫羊藿花粉形态对比研究［J］.广西植物，2020，40（9）：1300-1314.
	HUANG H， LAI H Q， JIANG Y，et al.Comparative study on pollen morphology of Epimedium sagittatum and its sibling species，E.myrianthum ［J］.Guihaia，2020，40（9）：1300-1314.
20	PUNT W.Pollen morphology of the dichapetalaceae with special reference to evolutionary trends and mutual relationships of pollen types［J］.Review of Palaeobotany and Palynology，1975，19（1）：1-97.
21	杨向晖，吴颖欣，林顺权.6种枇杷属植物花粉形态扫描电镜观察［J］.果树学报，2009，26（4）：572-576.
	YANG X H， WU Y X， LIN S Q.SEM observation on the pollen morphology of six Eriobotrya plants［J］.Journal of Fruit Science，2009，26（4）：572-576.
22	束际林，陈亮，王海思，等.茶树及其他山茶属植物花粉形态、超微结构及演化［J］.茶叶科学，1998，18（1）：6-15.
	SHU J L， CHEN L， WANG H S，et al.Pollen morphology，ultrastructure and evolution of tea plant and other genus Camellia plants［J］.Journal of Tea Science，1998，18（1）：6-15.
23	LU K Q， LI M， WANG G H，et al.New pollen classification of Chenopodiaceae for exploring and tracing desert vegetation evolution in eastern arid central Asia［J］.Journal of Systematics and Evolution，2018，57（2）：190-199.
24	吕林玲.后生山茶亚属（山茶科）植物分类学研究［D］.厦门：厦门大学，2015：42-51.
	LÜ L L.Taxonomic study on the subgen.Metacamellia（Theaceae）［D］.Xiamen：Xiamen University，2015：42-51.
25	江正栋.基于叶绿体DNA的山茶属植物的分子系统学和生物地理学初探［D］.杭州：浙江理工大学，2017：17-31.
	JIANG Z D.Preliminary study of molecular phylogenetics and biolgeography of the genus Camellia L. based on chloroplast DNA［D］.Hangzhou：Zhejiang Sci-Tech University，2017：17-31.

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