Abstract: Using inter-simple sequence repeat (ISSR) makers, genetic diversity and genetic differentiation of Quercus glandulifera var. brevipetiolata populations, which distribute in Zhejiang Province, were analyzed. A total of 132 bands were amplified from 7 populations with 140 individulas by 12 reliable primers, which was selected from 100 primers. Of them, 118 bands were polymorphic and the percentage of polymorphic loci of Quercus glandulifera var. brevipetiolata species was 89.39%. The percentage of polymorphic loci of Quercus glandulifera var. brevipetiolata populations averaged 58.87%. Shannon information index (I) and Nei’s gene index (h) at species level were 0.493 3 and 0.334 7, respectively, while those at population level averged 0.336 2 and 0.229 1, respectively. It suggested that the genetic diversity of YF population was the highest while that of TTS population was the lowest. Analysis of molecular various showed that 67.97% of the total variation was distributed among populations, while only 32.03% was distributed among individuals within populations. The gene differentiation coefficient (G_ST) among populations is 0.315 4. The gene flow among populations is 1.085 3. The average genetic distance of 7 populations was 0.173 9. Using unweighted pair group method arithmatic average (UPGMA), TTS population and XDS population were clustered into one group and the other five populations were clustered into another group.

Key words: Quercus glandulifera var. brevipetiolata, Genetic diversity, Genetic differentiation, ISSR