Abstract: T-DNA, have been cloned in plasmid vector pBTC-8, were trasferred into cucumber by introducing of Ri-plasmid.Explants of cucumber were cocultured with Agrobacterium rhizogenes harboring the binary vector, and the transfomants with typical phenotype of hairy roots were yielded.The transformed-roots were moved on a culture medium containing Kanamycin to induce the calli and shoots derivated from the calli.Detection of opine showed that the regene rated-shoots are transferred by Ri-plasmid.Transgenic plant show up response to Kanamycin.

Key words: Ri-plasmid, Transformation, Cucumber