Abstract: It was studies that the mains factor in fluencing rooting and transplanting of tissue cultured shoots of Toona sinensis. The results were summarized as follows:the main factor influencing rooting of tissue cultured shoots of Toona sinensis is IBA, and the contents of macronutrients in MS medium come second. The proper rooting medium is single IBA 1.0mg/L in MS medium without macronu-tients. The rooting rates was lowly with high concentration of NAA or IBA in a short time. The proper transplanting period is from March to early July every year. Vermiculites or sands is a good transplanting medium. The key to ensure successful transplantation is exercising plants effectively, proper transplanting medium and time. At the same time it is also important that preventing insects, shading and keeping air humidity.

Key words: Toona sinensis, tissue cultured shoots, rooting, transplantation