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Bulletin of Botanical Research ›› 2006, Vol. 26 ›› Issue (2): 201-205.doi: 10.7525/j.issn.1673-5102.2006.02.018

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In-vitro regeneration of Populus langfangensis 3 for transformation and micropropagation

WU Shuang-Xiu;ZU Yuan-Gang   

  1. 1. Department of Biology, Shanghai Normal University, Shanghai 200234 2. Key Laboratory of Forest Plant Ecology of the Ministry of Education, Northeast Forestry University, Harbin 150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-03-20 Published:2006-03-20
  • Contact: WU Shuang-Xiu
  • Supported by:

Abstract: The direct and indirect induction of organ differentiation, callus formation and plantlet regeneration from the leaf and stem explants of the hybrid Populus langfangensis 3 (P. deltoides (“Shan Hai Guan”)×((P. simonii × pyramidalys)12×Ulmus pumila) were investigated in-vitro on Murashige and Skoog (MS) medium supplemented with various concentrations of benzylaminopurine (BA), indole-3-butyric acid (IBA), 1-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). The petiole and leaf explant was easier for direct shoot regeneration along the veins at cutting sites. Direct shooting efficiency was related to both BA concentration and BA/IBA ratio. Efficient and rapid shooting from the leaf and stem explants was achieved on MS medium containing 1~2 mg·L-1 BA and 0.5 mg·L-1 IBA. Maximum shooting frequency reached 90%. 2,4-D promoted the callus induction of P. langfangensis 3. The shooting based on callus was obtained on MS medium supplemented with 0.3~0.5 mg·L-1 BA and 0.02 mg·L-1 IBA or NAA and the corresponding shooting frequency was 76%. Rooting based on callus succeeded on 0.1 mg·L-1 BA and 0.2~0.5 mg·L-1 IBA, yielding rooting frequency 67%. These regeneration conditions could further be used for micropropagation and transformation of P. langfangensis 3.

Key words: Populus langfangensis 3, shooting, rooting, regeneration, callus formation

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