Abstract: The leaves of Eucommia ulmoides Oliv. L33 as explants, the B5 being as the basic medium supplemented with auxin, the combination of auxin and cytokinin, the callus induction and growth of E. ulmoides Oliv. were studied. The results showed that, callus wasn’t formed on the no phytohormones media. Callus was induced when explants were cultured on B5 medium supplemented with 2,4-D,NAA and IBA only. 0.5 mg·L^-1 2,4-D, 0.5~1.0 mg·L^-1 NAA, 1.0 mg·L^-1 IBA were induced 100 per cent for callus induction, and was good for the growth; When the cytokinin was added in the proper auxin medium, KT was bad for callus induction and growth; The best combination of callus induction and growth was 1.0 mg·L^-1 NAA+0.3~0.5 mg·L^-1 BA or 1.0 mg·L^-1 IBA+0.3~0.5 mg·L^-1 BA, whose calluses were growth quickly, suitable for subculture.

Key words: Eucommia ulmoides Oliv, tissue culture, callus induction, phytohormones