Abstract: The optimum macroporous resin and process were optimized for the purification of total flavonoids from Euonymus alatus，orthogonal-test method was adopted to study the effects of several factors including the kinds of resins, the concentration of eluent, the pH, etc., on the purification. The concentration of flavonoids in E. alatus was determined by ultraviolet spectrophotometry,the adsorption capacity, the rate of desorption and the content of total flavonoids in the extract were calculated to determine the suitable conditions. AB-8 resin is suitable for enriching and purifying total flavonoids of E. alatus, the optimum adsorption capacity and the rate of desorption are 7.32 mg·g^-1 and 90.98% respectively. The content of total in the extract was increased from 764% to 52.12%. This resin can be used to enrich and purify the total flavonoids of E. alatus, which can increase the content of total flavonoids in the extract.

Key words: Euonymus alatus, macroporous resin, orthogonal-test, purification