Expression Characteristics and Functional Analysis of the Jujube Gene ZjEB1c in Fruit Shape Regulation

doi:10.7525/j.issn.1673-5102.2026.05.011

Abstract

Abstract:

To clarify the role of the jujube（Ziziphus jujuba） EB1 family member ZjEB1c in fruit shape formation， EB1 family members in the Jingzao 39 genome were identified， and their expression patterns were analyzed across seven developmental stages in seven jujube cultivars. Meanwhile， an overexpression vector of ZjEB1c was constructed and heterologously transformed into tomato（Solanum lycopersicum）， and its regulatory effects were investigated through fruit phenotypic observation and transcriptome sequencing. The results showed that two EB1 family members， ZjEB1a and ZjEB1c， were identified in jujube. ZjEB1c was upregulated during the middle stage of fruit development（50-78 days post-anthesis）， and its expression level was higher in long-fruit cultivars. Compared with the wild type， the average fruit shape index of three independent transgenic tomato lines increased from 0.928 to 1.117-1.168， and the number of mesocarp cells per unit area increased significantly at the longitudinal base. 744 commonly differentially expressed genes were identified through comparative transcriptome analysis， which were mainly enriched in hormone signal transduction， cell wall and pectin metabolism-related pathways. Comprehensive analysis indicated that ZjEB1c was closely associated with fruit longitudinal elongation and suggested that it participated in fruit shape formation through the processes related to hormone signal transduction and cell division.

Key words: jujube, fruit shape, microtubule-associated protein, ZjEB1c

CLC Number:

S665.1

Wenqing FU, Rongshen MA, Yue REN, Zimeng LI, Xu WANG, Wenhao BO. Expression Characteristics and Functional Analysis of the Jujube Gene ZjEB1c in Fruit Shape Regulation[J]. Bulletin of Botanical Research, 2026, 46(5): 899-912.

Figures/Tables 6

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编号 Number	引物名称 Primer name	序列（5′→3′） Sequence（5′→3′）
1	ZjEB1c-F	ATGGCGACGAACATTGGTATG
2	ZjEB1c-R	CTAGTAGGTCATAAGAGCTG
3	pBI121-ZjEB1c-F	GAGAACACGGGGGACTCTAGATGGCGACGAACATTGTATGATGGATTC
4	pBI121-ZjEB1c-R	GGGAAATTCGAGCTCGGTACCTAGTAGGTCATAAGAGCTGATCCACTG
5	35S	TTCATTTGGAGAGAACACGGGGGAC
6	NOS	CAAGACCGGCAACAGGATTCAATC

编号 Number	基因ID Gene ID	皮尔逊相关系数 Pearson correlation coefficient	推测功能 Putative function
1	101259852	0.999 089	内切葡聚糖酶16 Endoglucanase 16
2	101253063	0.999 089	色氨酸N-单加氧酶CYP79A68样蛋白 Tryptophan N-monooxygenase CYP79A68-like
3	101260874	0.994 657	核糖核酸酶3样蛋白2样蛋白 Ribonuclease 3-like protein 2-like
4	101256020	0.993 607	丝氨酸/苏氨酸蛋白激酶STY17 Serine/threonine-protein kinase STY17
5	543580	0.993 285	ER6蛋白 ER6 protein
6	101257490	0.992 775	蛋白转运蛋白SEC16B同源蛋白 Protein transport protein SEC16B homolog
7	101262229	-0.992 040	可能的6-磷酸葡萄糖酸内酯酶4（叶绿体） Probable 6-phosphogluconolactonase 4，chloroplastic
8	101258554	0.992 026	受体样蛋白14 Receptor-like protein 14
9	101055571	0.991 905	钙调蛋白结合转录因子SR1L Calmodulin-binding transcription factor SR1L
10	101264910	-0.990 710	30S核糖体蛋白S17（叶绿体） 30S ribosomal protein S17，chloroplastic
11	101258235	0.988 833	I型肌醇多磷酸5-磷酸酶12样蛋白 Type I inositol polyphosphate 5-phosphatase 12-like
12	101246721	0.988 804	Scarecrow样蛋白14 Scarecrow-like protein 14