Bulletin of Botanical Research ›› 2022, Vol. 42 ›› Issue (6): 976-985.doi: 10.7525/j.issn.1673-5102.2022.06.007
• Molecular biology • Previous Articles Next Articles
Ting QIAN, Fan ZHAO, Yujie ZHANG, Xueli LI, Kun SUN, Hui ZHANG()
Received:
2021-11-03
Online:
2022-11-20
Published:
2022-11-22
Contact:
Hui ZHANG
E-mail:zhanghui@nwnu.edu.cn
About author:
QIAN Ting(1995—),female,master,major in plant systematics and evolution.
Supported by:
CLC Number:
Ting QIAN, Fan ZHAO, Yujie ZHANG, Xueli LI, Kun SUN, Hui ZHANG. Study on Adaptive Differentiation of Transcription Factor bHLH94 Gene to Altitude in Hippophae neurocarpa and H.tibetana[J]. Bulletin of Botanical Research, 2022, 42(6): 976-985.
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URL: https://bbr.nefu.edu.cn/EN/10.7525/j.issn.1673-5102.2022.06.007
Table 1
Collection information of Hippophae
物种 Species | 采集地 Location | 样品编号 Code. No | 海拔 Altitude /m |
---|---|---|---|
肋果沙棘 H. neurocarpa | 青海省果洛州达日桥头 Dari Bridge Head,Goluo Prefecture,Qinghai Province | N_H | 3 895 |
青海省野牛沟乡大浪村 Dalang Village,Ye niugou Township,Qinghai Province | N_M | 3 533 | |
青海省祁连县八宝镇冰沟村 Binggou Village,Babao Town,Qilian County,Qinghai Province | N_ML | 3 352 | |
青海省祁连县日旭村 Rixu Village,Qilian County,Qinghai Province | N_L | 3 188 | |
西藏沙棘 H. tibetana | 青海省果洛州达日县东日寺 Dongri Temple,Dari County,Guoluo Prefecture,Qinghai Province | T_H | 4 066 |
青海省野牛沟乡大浪村 Dalang Village,Yeniugou Township,Qinghai Province | T_M | 3 533 | |
青海省祁连县日旭村 Rixu Village,Qilian County,Qinghai Province | T_L | 3 188 |
Table 2
Sources of protein sequence data from the key nodes of the phylogenetic tree of plants
物种 Species | NCBI物种编号 Number of species in NCBI | 数据来源 Data sources | 蛋白序列版本 Version of protein sequence |
---|---|---|---|
Amborella trichopoda | txid13333 | https://phytozome.jgj.doe.gov/pz/portal.html | v1.0 |
Arabidopsis thaliana | txid3702 | https://phytozome.jgj.doe.gov/pz/portal.html | TAIR10 |
Zea mays | txid4577 | https://phytozome.jgj.doe.gov/pz/portal.html | v1.1 |
Helianthus annuus | txid4232 | https://phytozome.jgj.doe.gov/pz/portal.html | r1.2 |
Solanum lycopersicum | txid4081 | https://phytozome.jgj.doe.gov/pz/portal.html | ITAG2.4 |
Oryza sativa | txid4530 | https://phytozome.jgj.doe.gov/pz/portal.html | v7.0 |
Physcomitrium patens | txid3128 | https://phytozome.jgj.doe.gov/pz/portal.html | v3.3 |
Selaginella moellendorffii | txid88036 | https://phytozome.jgj.doe.gov/pz/portal.html | v1.0 |
Picea abies | txid3329 | https://congenie.org/ | v1.0 |
Ziziphus jujuba | txid26968 | https://www.ncbi.nlm.nih.gov/ | 1.1 |
Trema orientale | txid63057 | https://www.ncbi.nlm.nih.gov/ | asm01 |
Parasponia andersonii | txid3476 | https://www.ncbi.nlm.nih.gov/ | asm01 |
Chlamydomonas reinhardtii | txid3055 | https://www.ncbi.nlm.nih.gov/ | v3.0 |
Hippophae neurocarpa | txid193523 | 本研究提供 Provied from this research | |
Hippophae tibetana | txid193519 | 本研究提供 Provied from this research |
Table 3
Primers used in PCR and qRT-PCR
引物名称 Primer name | 引物序列 Primer sequence(5′—3′) |
---|---|
HnbHLH94 | F:CAGTTATGGCGGAAATGAGC R:CACAGCATCAAGAGCAGACAAC |
HtbHLH94 | F:GTCAGTTATGGCGGAAACGAG R:ACCACAGCATCAAGAGCAGAC |
qRT-HnbHLH94 | F:CTCCTCCACCTCTTCACCTCCTTC R:TCCAGCGTTTGTTGGGTCTGTTTC |
qRT-HtbHLH94 | F:CCACCTCTTCACCTCCTTCAATGG R:CAGCGTTTGTTGGGTCTGTTTCAG |
Actin | F:GCAGCAGGTAGTTGAGGATT R:AAGAAGGGCAGAGAACATGAG |
Table 4
Statistics of the assembly results of unigenes of H. neurocarpa and H. tibetana
样品 Sample | 200~500 bp | 500~1 kbp | 1 k~2k bp | >2 kbp | 合计 Total |
---|---|---|---|---|---|
N_L | 191 173 | 29 975 | 12 141 | 6 310 | 239 599 |
N_ML | 158 804 | 26 688 | 11 272 | 6 342 | 203 106 |
N_M | 183 449 | 28 847 | 11 833 | 6 218 | 230 347 |
N_H | 151 730 | 24 374 | 10 996 | 5 853 | 192 953 |
T_L | 155 163 | 28 033 | 12 361 | 6 689 | 202 246 |
T_M | 230 291 | 41 293 | 17 601 | 8 177 | 297 362 |
T_H | 151 032 | 26 931 | 11 340 | 6 269 | 195 572 |
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