Quantitative Analysis of Leaf Photosynthetic Heterogeneity Based on Chlorophyll Fluorescence Imaging and Distribution Characteristics of Fluorescence Parameters

doi:10.7525/j.issn.1673-5102.2022.06.015

Abstract

Abstract:

Leaf photosynthesis has high spatial heterogeneity， and chlorophyll fluorescence imaging technology provides convenience for the study of leaf photosynthetic heterogeneity， however， the analytical method for quantifying photosynthetic heterogeneity has not been widely used yet. In this study， the chlorophyll fluorescence images for the sunny and shady leaves of Ficus microcarpa in the mid subtropical region during overwintering period were obtained by using the PAM chlorophyll fluorescence imaging system（Walz， Germany）， and the photosynthetic heterogeneity characteristics of sunny and shady leaves were quantitatively analyzed respectively. The results showed that the degree of photosynthetic heterogeneity and photoinhibition of sunny leaves were significantly higher than those of shady leaves for F. microcarpa during overwintering， and the coefficient of variation（C_V） could be used as a quantitative index of photosynthetic heterogeneity. For sunny leaves 4.30% necrotic areas， 53.30% severe photoinhibition areas（0<F_v/F_m<0.627） and 42.27% mild photoinhibition leaf areas（0.627≤F_v/F_m<0.800） were observed under low temperature but high light condition， respectively. On the other hand， for shady leaves lesser photoinhibition and leaf necrosis were observed under low temperature and low light condition. Furthermore， the quantitative analysis of other fluorescence parameters， such as effective quantum yield of photosynthetic system Ⅱ（PSⅡ） photochemistry（Y（Ⅱ））， quantum yield of regulated energy dissipation（Y（NPQ）） and quantum yield of non-regulated energy dissipation （Y（NO））， showed that the sunny leaves possessed relatively higher photochemical ability， while the shady leaves had relatively higher heat dissipation capacity. In addition， although in winter the high light would lead to the serious accumulation of excitation pressure of the PSⅡ in sunny leaves， which indicated potential risk of serious photoinhibition stress， the necrotic areas were not large， and there were still some low-risk areas with low excitation pressure in sunny leaves. However， the shady leaves under low temperature and low light were mainly low-risk areas with low excitation pressure.

Key words: chlorophyll fluorescence imaging technology, photosynthetic heterogeneity, photoinhibition, Ficus microcarpa

CLC Number:

Q945.79

Wenhai HU, Yi’an XIAO. Quantitative Analysis of Leaf Photosynthetic Heterogeneity Based on Chlorophyll Fluorescence Imaging and Distribution Characteristics of Fluorescence Parameters[J]. Bulletin of Botanical Research, 2022, 42(6): 1052-1061.

Figures/Tables 8

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叶片类型 Types of leaf	样本	C_V
叶片类型 Types of leaf	样本	α=0.004	α=0.02	α=0.04
阳生 Sun-leaf	叶1 Leaf-1	0.246	0.245	0.246
	叶2 Leaf-2	0.208	0.207	0.207
	叶3 Leaf-3	0.265	0.264	0.264
	均值 Average	0.240±0.017a	0.239±0.017a	0.239±0.017a
阴生 Shade-leaf	叶1 Leaf-1	0.094	0.094	0.094
	叶2 Leaf-2	0.071	0.071	0.071
	叶3 Leaf-3	0.063	0.063	0.063
	均值 Average	0.076±0.009b	0.076±0.009b	0.076±0.009b

区间间隔 Interval of fluorescence value （a）	0.004		0.02		0.04
叶片类型 Types of leaf	阳生叶 Sun-leaf	阴生叶Shade-leaf	阳生叶 Sun-leaf	阴生叶 Shade-leaf	阳生叶 Sun-leaf	阴生叶 Shade-leaf
P_{ΔSt max}荧光值（比例） Fluorescence value with max P_{ΔSt max} （Proportion）	0.667 （4.87%）	0.725 （3.96%）	0.667 （10.72%）	0.725 （18.12%）	0.667 （21.44%）	0.745 （36.11%）
P_ΔSt>1荧光值区间（比例） The range of fluorescence value with P_ΔSt>1 （Proportion）	非连续区间	0.651~0.776 （88.5%）	0.510~0.745 （92.8%）	0.627~0.784 （95.4%）	0.510~0.784 （94.2%）	0.627~0.784 （96.3%）
P_ΔSt>10荧光值区间（比例） The range of fluorescence value with P_ΔSt>10 （Proportion）	—	—	非连续区间	0.686~0.765 （77.4%）	0.549~0.706 （65.2%）	0.667~0.784 （93.5%）
坏死区（F_v/F_m=0）比例 Proportion of leaf necrosis（F_v/F_m=0）	4.30%	0.09%	4.30%	0.09%	4.30%	0.09%
重度光抑制区（0<F_v/F_m<0.627）比例 Proportion of severe photoinhibition areas （0<F_v/F_m<0.627）	53.30%	4.61%	44.38%	3.19%	33.96%	2.31%
轻度光抑制区（0.627≤F_v/F_m<0.800）比例 Proportion of mild photoinhibition areas （0.627≤F_v/F_m<0.800）	42.27%	94.71%	51.12%	95.42%	61.53%	96.31%
健康区（F_v/F_m≥0.800）比例 Proportion of healthy areas（F_v/F_m≥0.800）	0.13%	0.59%	0.20%	1.29%	0.20%	1.29%

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