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Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (6): 896-903.doi: 10.7525/j.issn.1673-5102.2021.06.007

• Research Report • Previous Articles     Next Articles

Phylogenetic Analysis of DNA Barcoding Sequences in the Genus Salix

Wei-Chao REN1, Jiao XU2, Wei SUN3, Mei-Qi LIU1, Xin-Xin YU1, Si-Jia WANG1, Wei MA1()   

  1. 1.Pharmacy College,Heilongjiang University of Chinese Medicine,Harbin 150040
    2.Jiamusi College,Heilongjiang University of Chinese Medicine,Jiamusi 154007
    3.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700
  • Received:2020-11-13 Online:2021-11-20 Published:2021-10-29
  • Contact: Wei MA E-mail:88788891@qq.com
  • About author:REN Wei-Chao(1989—),male,doctor,engaged in development and utilization of traditional Chinese medicine resources.
  • Supported by:
    National Chinese Medicine Resources survey Project of the State Administration of Traditional Chinese Medicine(GZY-KJS-2018-004);Outstanding Innovative Talents Leading Talent Project of Heilongjiang University of Traditional Chinese Medicine(2018RCL09)

Abstract:

We used phylogenetic analysis to compare the ITS2 and psbA-trnH sequences of the plant of the genus Salix samples, so as to explore the genetic diversity and genetic relationship of these resources. Extract genomic DNA of the genus Salix and Tamarix chinensis samples. The ITS2 and psbA-trnH gene sequences of each samples were amplified and sequenced by PCR. The intraspecific and interspecific genetic distances and the neighbor joining(NJ) methods were used to construct phylogenetic trees. According to the phylogenetic tree of ITS2. S. pentandraS. roridaS. linearistipularis and S. schwerinii formed a monophyletic clade, distinguished from the same genus. Phylogenetic tree constructed by psbA-trnH sequences were unable to distinguish the genus Salix samples from different geographical sources. Therefore, ITS2 sequence was suitable for the authentication, and would provide a certain reference for the subsequent development and research of the plant of genus Salix.

Key words: Salix, ITS2, psbA-trnH, DNA barcoding, molecular identification

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