Extraction of Camptothecin and Hydroxyl Camptothecin from Camptothecin Leaves by Micellar Mediation Combined with Ultrasound-Microwave-Assisted Method

doi:10.7525/j.issn.1673-5102.2020.06.018

Abstract

Abstract:

In order to explore a new method to extract camptothecin and hydroxy-camptothecin from camptothecin leaves and make full use of camptothecin resources， the active ingredients of camptothecin and hydroxy-camptothecin from camptothecin leaves were extracted from camptothecin leaves powder as raw material. In this paper， the influence of microwave processing time， solid-liquid ratio， microwave power and the concentration of surfactant on the total extraction rate were investigated respectively， and the optimum extraction conditions were obtained. The results showed that the optimal extraction rate was achieved when the microwave processing time was 10 min 30 sec， the solid-liquid ratio was 1∶60， the microwave power was 850 W， and the concentration of surfactant was 8.5 g·L^-1. The total extraction rate was 0.056 4% under the optimal process.

Key words: camptothecin, hydroxyl camptothecin, surfactant, ultrasound-microwave-assisted method

CLC Number:

R914.4

Jian-Hang YANG, Xiu-Hua ZHAO. Extraction of Camptothecin and Hydroxyl Camptothecin from Camptothecin Leaves by Micellar Mediation Combined with Ultrasound-Microwave-Assisted Method[J]. Bulletin of Botanical Research, 2020, 40(6): 951-960.

Figures/Tables 16

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水平 Level	因素Factors
水平 Level	微波时间 Microwave time（min）	料液比 Solid-liquid ratio	微波功率 Microwave power（W）	表面活性剂浓度 Surfactant concentration （g·L^-1）
-1	8.00	1:40	700	6.00
0	10.00	1:60	850	8.00
1	12.00	1:80	1000	10.00

实验序号 No.	微波时间 Microwave time （min）	料液比 Material liquid than	微波功率 Microwave power （W）	表面活性剂浓度 Surfactant concentration （g/L）	总提取率 The total extraction yield (%)
1	8.00	40.00	850.00	8.00	0.04019
2	12.00	40.00	850.00	8.00	0.05028
3	12.00	60.00	1000.00	8.00	0.05001
4	10.00	40.00	850.00	10.00	0.05056
5	12.00	60.00	700.00	8.00	0.05101
6	10.00	80.00	850.00	10.00	0.04978
7	8.00	60.00	850.00	10.00	0.05019
8	8.00	80.00	850.00	8.00	0.05117
9	10.00	60.00	850.00	8.00	0.05782
10	8.00	60.00	1000.0	8.00	0.05005
11	10.00	60.00	700.00	10.00	0.05023
12	10.00	60.00	850.00	8.00	0.05963
13	10.00	60.00	850.00	8.00	0.05418
14	10.00	40.00	850.00	6.00	0.03963
15	10.00	60.00	700.00	6.00	0.04113
16	10.00	60.00	850.00	8.00	0.05211
17	10.00	80.00	700.00	8.00	0.05048
18	10.00	80.00	850.00	6.00	0.05014
19	12.00	60.00	850.00	10.00	0.05037
20	12.00	80.00	850.00	8.00	0.05028
21	10.00	40.00	700.00	8.00	0.04027
22	10.00	60.00	850.00	8.00	0.05351
23	10.00	40.00	1000.00	8.00	0.05113
24	8.00	60.00	850.00	6.00	0.03997
25	10.00	60.00	1000.00	10.00	0.05091
26	8.00	60.00	700.00	8.00	0.04017
27	12.00	60.00	850.00	6.00	0.05031
28	10.00	80.00	1000.00	8.00	0.04983
29	10.00	60.00	1000.00	6.00	0.05014

来源 Source	平方和 Sum of square	自由度 Degrees of freedom	均方 Mean squre	F值 F-value	P值 P-value
Model	0.131 7	14	0.034 4	15.47	＜0.000 1
A微波时间Microwave time	0.064 1	1	0.064 1	28.84	＜0.000 1
B料液比Solid-liquid ratio	0.048 9	1	0.048 9	22.00	0.000 3
C微波功率Microwave power	0.046 2	1	0.046 2	20.77	0.000 4
D表面活性剂浓度Surfactant concentration	0.064 5	1	0.064 5	28.97	＜0.000 1
AB	0.020 1	1	0.020 1	9.07	0.009 3
AC	0.019 8	1	0.019 8	8.91	0.009 9
AD	0.007 9	1	0.007 9	3.57	0.079 7
BC	0.022 1	1	0.022 1	9.97	0.007 0
BD	0.0213	1	0.021 3	9.59	0.007 9
CD	0.011 6	1	0.011 6	5.22	0.038 4
A²	0.054 3	1	0.055 1	24.40	0.000 2
B²	0.018 9	V	0.018 9	31.10	＜0.000 1
C²	0.062 3	1	0.062 3	28.03	0.000 1
D²	0.056 8	1	0.054 26	25.51	0.000 2
残差Residual	0.031 1	14	0.007 9
失拟项Lack of Fit	0.016 5	10	0.006 2	0.070	0.999 6
误差Pure Error	0.026 5	4	0.023 7
总计Cor Total	0.139 7	28

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