Molecular Phylogenetic Analysis of Cerasus Plants in China

doi:10.7525/j.issn.1673-5102.2020.06.010

Abstract

Abstract:

To provide some molecular basis for the species classification and lineage location of cherry blossoms mainly planted in China， with 88 cherry blossoms， by nuclear gene ITS and chloroplast intergenic sequence， trnH-psbA were sequenced， and after processed by software MEGA 6， phylogenetic tree was constructed. The phylogenetic development was confused in Cerasus serrulata group， which is related to the complex genetic background and the close phylogenetic relationship among the primitive parental， meanwhile， it could be clustered together in most of the cultivars C.subhirtella group or C.campanulata group， and could be separated from the cultivars of the Cerasus serrulata group， however， it was difficult to distinguish in many hybrids using ITS and trnH-psbA， which indicated that DNA barcoding technology was difficult to apply to the phylogenetic relationship of hybrids， in addition， some cherry cultivars could be identified by ITS or trnH-psbA sequence length， and some unidentified varieties were also revised.

Key words: Cerasus, ITS, chloroplast sequence, phylogenetic, species identification

CLC Number:

Q341

Tao FU, Zhi-Long WANG, Le-Jing LIN, Li LIN, Wen LI, Dong-Ming YUAN. Molecular Phylogenetic Analysis of Cerasus Plants in China[J]. Bulletin of Botanical Research, 2020, 40(6): 876-885.

Figures/Tables 5

Table 1

Samples information and source of the 88 cherry blossoms

序号No.	物种Species	来源Source
山樱群Cerasus serrulata group
1	青肌樱Cerasus Multiplex	原产中国 Native to China
2	仙台屋樱C.jamasakura‘Sendaiya’	红山樱系园艺栽培品种Horticultural cultivar of C.jamasakura series
3	内里樱C.jamasakura‘Imperialis’	红山樱系园艺栽培品种Horticultural cultivar of C.jamasakura series
4	市原虎尾樱C.jamasakura‘Albo Plena’	红山樱系园艺栽培品种Horticultural cultivar of C.jamasakura series
5	红叶樱C.serrulata‘Hongye’	山樱系园艺栽培品种Horticultural cultivar of C.serrulata series
6	奈良八重樱C.verecunda‘Antiqua’	霞樱系重瓣品种Double variety of C.verecunda series
7	大岛樱C.speciosa	日本原生种 Japanese native species
8	伊豆樱C.speciosa‘Izuzakura’	大岛樱变异品种Variation variety of C.speciosa
9	赤实大岛樱C.speciosa‘Akami-ohshima’	大岛樱系园艺栽培品种Horticultural cultivar of C.speciosa series
10	八重红大岛樱C.speciosa‘Yaebeni-ohshima’	大岛樱系园艺栽培品种Horticultural cultivar of C.speciosa series
11	白花真樱C.serrulata‘Multiplex’	日本晚樱×樱桃 C.serrulata var. lannesiana×C.pseudocerasus
12	白妙樱C.serrulata‘Sirotae’	真樱影响的日本晚樱C.serrulata var.lannesiana influenced by C.serrulata
13	千里香樱C.serrulata‘Senriko’	真樱影响的日本晚樱C.serrulata var. lannesiana influenced by C.serrulata
14	太白樱C.serrulata‘Taihaku’	真樱影响的日本晚樱C.serrulata var. lannesiana influenced by C.serrulata
15	雨宿樱C.serrulata‘Amayadori’	真樱影响的日本晚樱C.serrulata var. lannesiana influenced by C.serrulata
16	大提灯樱C.serrulata‘Ojochin’	真樱影响的日本晚樱C.serrulata var. lannesiana influenced by C.serrulata
17	兰兰樱C.serrulata‘Ranran’	真樱影响的日本晚樱（白兰×雨宿） C.serrulata var.lannesiana influenced by C.serrulata（ハクラン×C.serrulata‘Amayadori’）
18	兼六园菊樱C.serrulata‘Sphaerantha’	山樱大山樱影响的日本晚樱C.serrulata var. lannesiana influenced by C.serrulata and C.sargentii
19	关山樱C.serrulata‘Kanzan’	山樱大山樱影响的日本晚樱C.serrulata var.lannesiana influenced by Cserrulata and Csargentii
20	大村樱C.serrulata‘Mirabilis’	山樱大山樱影响的日本晚樱C.serrulata var.lannesiana influenced by C.serrulata and C.sargentii
21	红华樱C.serrulata‘Kouka’	山樱大山樱影响的日本晚樱（大山樱× 日本晚樱） C.serrulata var. lannesiana influenced by C.serrulata and C.sargentii（C.sargentii×C.serrulata var.lannesiana）
22	手弱女樱C.serrulata‘Taoyame’	山樱大山樱影响的日本晚樱（大岛×山樱） C.serrulata var. lannesiana influenced by C.serrulata and C.sargentii（C.speciosa×C.serrulata）
23	梅护寺数珠挂樱C.serrulata‘Juzukakezakura’	山樱大山樱影响的日本晚樱C.serrulata var. lannesiana influenced by C.serrulata and C.sargentii
24	山樱枝垂樱C.serrulata‘Sendai-shidare’	山樱大山樱影响的日本晚樱（大岛×未知） C.serrulata var. lannesiana influenced by C.serrulata and C.sargentii（C.speciosa×Unknow）
25	鹎樱C.serrulata‘Longipedunculata’	霞樱影响的日本晚樱C.serrulata var. lannesiana influenced by C.verecunda
26	菊垂樱C.serrulata‘Plena-pendula’	霞樱影响的日本晚樱C.serrulata var. lannesiana influenced by C.verecunda
27	红玉锦樱C.serrulata‘Beni-tamanishiki’	霞樱影响的日本晚樱（八重霞樱×日本晚樱） C.serrulata var. lannesiana influenced by C.verecunda（C.verecunda f.plena×C.serrulata var.lannesiana）
28	松前红绯衣樱C.serrulata‘Matsumae-benihigoromei’	高岭樱影响的日本晚樱（高岭樱×日本晚樱） C.serrulata var. lannesiana influenced by C.nipponica（C.nipponica×C.serrulata var.lannesiana）
29	骏河台香樱C.speciosa‘Surugadai-odora’	大岛樱影响的日本晚樱C.serrulata var.lannesiana influenced by C.speciosa
30	御车返樱C.serrulata‘ Mikurumakaisi’	大岛樱影响的日本晚樱C.serrulata var.lannesiana influenced by C.speciosa
31	天川樱C.serrulata‘Erecta’	大岛樱影响的日本晚樱C.serrulata var.lannesiana influenced by C.speciosa
32	一叶樱C.serrulata‘Hisakura’	大岛樱影响的日本晚樱C.serrulata var.lannesiana influenced by C.speciosa
33	松月樱C.serrulata‘Superba’	大岛樱影响的日本晚樱C.serrulata var.lannesiana influenced by C.speciosa
34	普贤象樱C.serrulata‘Albo-rosea’	大岛樱影响的日本晚樱C.serrulata var.lannesiana influenced by C.speciosa
35	御衣黄樱C.serrulata‘Gioiko’	大岛樱影响的日本晚樱C.serrulata var. lannesiana influenced by C.speciosa
36	郁金樱C.serrulata‘Grandiflora’	大岛樱影响的日本晚樱C.serrulata var.lannesiana influenced by C.speciosa
37	日暮樱C.serrulata‘Amabilis’	大岛樱影响的日本晚樱C.serrulata var.lannesiana influenced by C.speciosa
38	苔清水樱C.serrulata‘Kokeshimidsu’	大岛樱影响的日本晚樱C.serrulata var. lannesiana influenced by C.speciosa
39	福禄寿樱C.serrulata‘Contorta’	福禄寿系的日本晚樱C.serrulata var. lannesiana influenced by C.serrulata‘Contorta’ systems
40	花笠樱C.serrulata‘Hanagasa’	福禄寿系的日本晚樱（“福禄寿”的实生苗） C.serrulata var.lannesiana influenced by C.serrulata‘Contorta’series（Seedling of C.serrulata‘Contorta’）
41	白雪樱C.serrulata‘Sirayuki’	白雪系的日本晚樱C.serrulata var.lannesiana influenced by C.serrulata‘Sirayuki’series
42	红笠樱C.serrulata‘Benigasa’	江户系园艺栽培品种（丝括×日本晚樱） Horticultural cultivar of C.serrulata‘Nobilis’ series（C.serrulata‘Fasciculata’×C.serrulata var.lannesiana）
43	杨贵妃樱C.serrulata‘Mollis’	江户系的日本晚樱C.serrulata var.lannesiana influenced by C.serrulata‘Nobilis’ series
44	红手毬樱 C.serrulata‘Benitemari’	江户系园艺栽培品种 Horticultural cultivar of C.serrulata‘Nobilis’ series
45	东锦樱C.serrulata‘Azuma-nishiki’	江户系的日本晚樱C.serrulata var. lannesiana influenced by C.serrulata‘Nobilis’ systems series
46	红时雨樱C.serrulata‘Beni-shigure’	江户系的日本晚樱（东锦的实生苗） C.serrulata var. lannesiana influenced by C.serrulata‘Nobilis’ series（Seedling of C.serrulata‘Azuma-nishiki’）
47	高砂樱C.× sieboldii‘Caepitosa’	高砂系的日本晚樱（丁字樱×日本晚樱） C.serrulata var.lannesiana influenced by C.×sieboldii‘Caepitosa’series（C.apetala×C.serrulata var.lannesiana）
48	红丰C.serrulata‘Beni-yutaka’	松前早咲×龙云院红八重C.serrulata‘Matsumae-hayazaki’ ×C.serrulata‘Ryuun-in-beniyae’
49	旭山.朝日山樱C.serrulata‘Asahiyama’	系统不确定日本晚樱System uncertainty of C.serrulata var.lannesiana
50	麒麟樱C.serrulata‘Kirin’	系统不确定日本晚樱System uncertainty of C.serrulata var.lannesiana
51	朱雀樱C.serrulata‘Shujaku’	系统不确定日本晚樱System uncertainty of C.serrulata var.lannesiana
52	白山2号樱Cerasus‘Hakusan-nigo’	系统不确定日本晚樱System uncertainty of C.serrulata var.lannesiana
53	弘前三段咲樱Cerasus‘Hirosaki-sandanzaki’	系统不确定日本晚樱System uncertainty of C.serrulata var.lannesiana
54	蝴蝶樱C.serrulata‘Kocho’	系统不确定日本晚樱System uncertainty of C.serrulata var.lannesiana
江户彼岸群Cerasus subhirtella group
55	江户彼岸樱C.subhirtella	日本原生种Japanese native species
56	八重红枝垂樱C.subhirtella‘Plena-rosea’	彼岸系园艺栽培品种Horticultural cultivar of C.subhirtella series
57	十月樱C.subhirtella‘Autumnalis’	（江户彼岸×豆樱）×小彼岸（C.subhirtella×C.incisa）×C.subhirtella‘Subhirtella’
58	神代曙樱C.yedoensis‘Jindai-akebono’	江户彼岸×未知C.subhirtella×Unknown
59	雨晴垂枝樱C.subhirtella‘Ujou-shidare’	彼岸系园艺栽培品种Horticultural cultivar of C.subhirtella series
60	小松乙女樱C.subhirtella‘Komatsu-otome’	彼岸系园艺栽培品种Horticultural cultivar of C.subhirtella series
61	越之彼岸樱C.subhirtella‘Koshinensis’	江户彼岸×大山樱C.subhirtella×C.sargentii
62	十月红樱C.subhirtella‘Autumnalis Rosea’	彼岸系园艺栽培品种Horticultural cultivar of C.subhirtella series
63	澳博拉大叶早樱C.subhirtella‘Ombrella’	彼岸系园艺栽培品种Horticultural cultivar of C.subhirtella series
64	思川樱C.subhirtella‘Omoigawa’	十月樱×未知C.subhirtella‘Autumnalis’×Unknown
65	红枝垂樱C.subhirtella‘Pendula Rosea’	彼岸系园艺栽培品种Horticultural cultivar of C.subhirtella series
66	染井吉野樱C.yedoensis	江户彼岸×大岛樱C.subhirtella×C.speciosa
67	嘉奖樱C.subhirtella‘Accolade’	大山樱×江户彼岸C.sargentii×C.subhirtella
68	小彼岸樱C.subhirtella‘Subhirtella’	豆樱×江户彼岸C.incisa×C.subhirtella
69	御帝吉野樱C.yedoensis‘Mikado-yoshino’	大岛樱×江户彼岸C.speciosa×C.subhirtella
70	潘多拉樱Cerasus‘Pandora’	大叶早樱某品种×染井吉野C.subhirtella‘Ascendens Rosea’×C.yedoensis
71	咲耶姬樱C.yedoensis‘Sakuyahime’	染井吉野实生苗Seedling of C.yedoensis
72	阳春樱Cerasus‘Yoshun’	亲本不详Unknown parent
寒绯樱群Cerasus campanulata group
73	寒绯樱C.campanulata	中国台湾原生种Taiwan of China native species
74	琉球寒绯樱C.campanulata‘Ryukyu-hizakura’	原产我国台湾及琉球群岛Originated in Taiwan and Ryukyu Islands of China
75	阳光樱C.campanulata‘Youkou’	天城吉野×寒绯樱C.yedoensis‘Amagi-yoshino’×C.campanulata
76	寒樱C.campanulata‘Kanzakura’	寒绯樱×山樱C.campanulata×C.serrulata
77	大寒樱C.campanulata‘Oh-kanzakura’	寒绯樱×大岛C.campanulata×C.speciosa
78	修善寺寒樱C.campanulata‘Rubescens’	寒绯樱×大岛C.campanulata×C.speciosa
79	才力樱C.campanulata‘Okame’	寒绯樱×豆樱C.campanulata×C.incisa
80	初美人樱C.campanulata‘Introrsa’	寒绯樱×樱桃C.campanulata×C.pseudocerasus
81	河津樱C.campanulata‘Kawazu-zakura’	寒绯樱×大岛樱C.campanulata×C.speciosa
82	横滨绯樱C.campanulata‘Kenrokuen-kumagai’	兼六园熊谷×寒绯樱C.jamasakura‘Kenrokuen-kumagai’×C.campanulata
83	大渔樱C.campanulata‘Tairyo-zakura’	早咲大岛樱×寒樱C.lannesiana var.speciosa×C.campanulata‘Kanzakura’
84	粉巨人樱Cerasus‘Pin giant’	亲本不详Unknown parent
豆樱群Cerasus incisa group
85	三岛富士樱C.incisa	日本原生种Japanese native species
86	阿丽亚娜富士樱C.incisa‘Ariane’	豆樱类品种Variety of C.incisa
87	维多利富士樱C.incisa‘Vitroeli’	豆樱类品种Variety of C.incisa
88	冬樱C.incisa‘Parvifolia’	豆樱×大岛樱C.incisa×C.speciosa

Table 1

Table 2

Table 3

Table 4

Fig. 1

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序列名称Sequence name	变异位点Variable
序列名称Sequence name	简约信息位点Parsim-info	单突变位点Singleton
ITS	A-C(41，190，414，509)，C-A(44，112，573)，C-T(63,145，163，184，205)，G-T(91，94，445)，A-G(96)，C-G(432)，T-G(438)，G-A(567)，A-T(580)	C-T(95，111，372，469，534，590),G-A(317)，G-C(502，559)，A-C(504，512)，T-G(502)
trnH-psbA	C-A(41)，G-T(281)	A-T(126，130)，T-A(133),C-A(300)

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