Effect of Soak Stems Overnight with Deionized Water on Measuring Maximum Hydraulic Conductivity in Xylem of Stem Segments of Robinia pseudoacacia L. and Age-relate Difference

doi:10.7525/j.issn.1673-5102.2020.05.009

Abstract

Abstract: The maximum hydraulic conductivity in xylem of branches is one of the core indexes in plant water physiology research. The method of flush the stem by perfusing solution at higher pressure which dissolves and expels the emboli is the most commonly used method which be used to measure the hydraulic conductivity in xylem of stem segments. However, it is necessary to find a reliable method to obtain stable maximum hydraulic conductivity prior to experiment.In this study, the differentyears branches of Robinia pseudoacacia L. was used as the experimental materials, and we compared the effect of two different sample treatment methods on measuring hydraulic conductivity in xylem of stem segments(i.e., without soaked stem segments overnight with deionized water and soaked stem segments overnight with deionized water prior to experiment), the reliability of the maximum hydraulic conductivity determination was determined by test whether the maximum hydraulic conductivity in xylem of stem segments can be kept constant with increasing flushing time, and explore the effective method to improve the stability of measuring maximum hydraulic conductivity of branches. Our results demonstrated that:①the water conductivity of the branches that had not been soaked overnight with deionized water decreased linearly with increasing flushing time after the maximum hydraulic conductivity was reached by 150 kPa pressure flushing, while the hydraulic conductivity of the branches that had been soaked overnight with deionized water prior to experiment kept constant by the same experimental method. ②The hydraulic conductivity of two-year-old branches kept constant after 150 kPa pressure flushing, regardless of whether they were soaked overnight with deionized water or not. However, after soaking stems overnight with deionized water, the time required for two-year-old branches to reach the maximum hydraulic conductivity was significantly shorter than that of non-soaking treatment. ③According to the percentage of xylem area on the cross section of stem and the wood density of branches, the lignification degree of current-year branches was significantly lower than that of biennial branches during the experiment. We speculate that the decrease with increasing flushing time of hydraulic conductivity in the current-year's branches without soaked overnight is due to the blockage of vessel lumen caused by the secretion produced by mechanical injury of branches, and that soaked stem segments overnight with deionized water prior to experiment can effectively reduce the possibility of secretion being introduced into vessel lumen. Therefore, the soaking stem segments overnight with deionized water prior to experiment can improve the stability of maximum hydraulic conductivity measuring.

Key words: Robinia pseudoacacia L., hydraulic conductivity in xylem of stem segments, soak with deionized water, flushing time, different age branches

CLC Number:

S792.26

LIANG Zhao, WEI Kai-Lu, YANG Dong-Mei, PENG Guo-Quan. Effect of Soak Stems Overnight with Deionized Water on Measuring Maximum Hydraulic Conductivity in Xylem of Stem Segments of Robinia pseudoacacia L. and Age-relate Difference[J]. Bulletin of Botanical Research, 2020, 40(5): 706-717.

References

[1] Sperry J S,Donnelly J R,Tyree M T.A method for measuring hydraulic conductivity and embolism in xylem[J].Plant,Cell & Environment,1988,11(1):35-40.
[2] Melcher P J,Holbrook N M,Burns M J,et al.Measurements of stem xylem hydraulic conductivity in the laboratory and field[J].Methods in Ecology and Evolution,2012,3(4):685-694.
[3] Giordano R,Salleo A,Salleo S,et al.Flow in xylem vessels and Poiseuille's law[J].Canadian Journal of Botany,1978,56(3):333-338.
[4] Jeje AA.The flow and dispersion of water in the vascular network of dicotyledonous leaves[J].Biorheology,1985,22(4):285-302.
[5] Zimmermann M H.Hydraulic architecture of some diffuse-porous trees[J].Canadian Journal of Botany,1978,56(18):2286-2295.
[6] Tyree M T,Graham M E D,Cooper K E,et al.The hydraulic architecture of Thuja occidentalis[J].Canadian Journal of Botany,1983,61(8):2105-2111.
[7] Ewers F W.Xylem structure and water conduction in conifer trees,dicot trees,and lianas[J].IAWA Bull NS,1985,6:309-317.
[8] Atkinson C J,Else M A,Taylor L,et al.Root and stem hydraulic conductivity as determinants of growth potential in grafted trees of apple(Malus pumila Mill.)[J].Journal of Experimental Botany,2003,54(385):1221-1229.
[9] Wang R Q,Zhang L L,Zhang S X,et al.Water relations of Robinia pseudoacacia L.:do vessels cavitate and refill diurnally or are R-shaped curves invalid in Robinia?[J].Plant,Cell &Environment,2014,37(12):2667-2678.
[10] Kelso W C Jr.,Gertjejansen R O,Hossfeld R L.The effect of air blockage upon the permeability of wood to liquids[Z].University of Minnesota Agricultural Research Station Technical Bulletin,1963:242.
[11] Jeje A A.The hydraulic conductivity of fresh wood:an isotropic,swelling,elasticoviscous porous medium[J].Biorheology,1986,23:40.
[12] 李荣,党维,蔡靖,等.6个耐旱树种木质部结构与栓塞脆弱性的关系[J].植物生态学报,2016,40(3):255-263.Li R,Dang W,Cai J,et al.Relationships between xylem structure and embolism vulnerability in six species of drought tolerance trees[J].Chinese Journal of Plant Ecology,2016,40(3):255-263.
[13] 冀艳利,郭印,陈建华,等.浙江师范大学校园药用植物资源及综合评价[J].浙江师范大学学报:自然科学版,2016,39(4):443-448.Ji Y L, Guo Y, Chen J H,et al.Resources and overall evaluation of medicinal plants in Zhejiang Normal University[J].Journal of Zhejiang Normal University:Natural Sciences,2016,39(4):443-448.
[14] Carrasco L O,Bucci S J,Di Francescantonio D,et al.Water storage dynamics in the main stem of subtropical tree species differing in wood density,growth rate and life history traits[J].Tree Physiology,2014,35(4):354-365.
[15] Anderson B E,Gortner R A,Schmitz H.Factors affecting the decreasing rate of flow of liquids through wood[Z].University of Minnesota Agricultural Research Station Technical Bulletin,1941,146.
[16] Buckman S J,Schmitz H,Gortnerr A.A study of certain factors influencing the movement of liquids in wood[J].The Journal of Physical Chemistry,2002,39(1):103-120.
[17] Krier J P.Factors causing decreasing rate of longitudinal flow of liquid through freshly cut woody stems[D].New Haven:Yale University,1951.
[18] 汪诗平.草原植物的放牧抗性[J].应用生态学报,2004,15(3):517-522.Wang S P.Grazing resistance of rangeland plants[J].Chinese Journal of Applied Ecology,2004,15(3):517-522.
[19] John W W,Curtis R W.Isolation and identification of the precursor of ethane in Phaseolus vulgaris L.[J].Plant Physiology,1977,59(3):521-522.
[20] Konze J R,Elstner E F.Ethane and ethylene formation by mitochondria as indication of aerobic lipid degradation in response to wounding of plant tissue[J].Biochimica et Biophysica Acta(BBA)-Lipids and Lipid Metabolism,1978,528(2):213-221.
[21] 李振国,魏佩珩,刘愚,等.植物遭受伤害后乙烯和乙烷产生的消长关系[J].植物生理学报,1982,18(4):335-342.Li Z G,Wei P H,Liu Y,et al.The inverse relationship between the production of ethylene and of ethane in plants after injury[J].Acta Phytophysiologia Sinica,1982,18(4):335-342.
[22] 史宏勇.臭椿茎中分泌道的形成及防御功能[D].西安:西北大学,2012.Shi H Y.The forming and defense function of secretory duct in the stem of Ailanthus altissima[D].Xi'an:Northwest University,2012.
[23] 王晓辉,孙永林,李广德,等.木本植物栓塞形成的原因及其生理作用研究进展[J].现代林业研究,2008,2(1):82-87.Wang X H,Sun Y L,Li G D,et al.Advances in research on inducing factors andphysiological action of embolism in woody plants[J].Journal of Modern Forestry,2008,2(1):82-87.
[24] Yang S F,Pratt H K.The physiology of ethylene in wounded plant tissue[C].//Kahl G.Biochemistry of Wounded Plant Tissue.Berlin:Walter de Gruyter,1978:595-622.
[25] Lieberman M.Biosynthesis and action of ethylene[J].Annual Review of Plant Physiology,1979,30:533-591.
[26] 刘愚,焦新之.植物体内乙烯的生物学作用及其调节控制[J].植物生理学报,1978,4(2):201-220.Liu Y,CHIAO S Z.Biological role of ethylene and ragulatary control of its production in plant[J].Acta Phytophysiologia Sinica,1978,4(2):201-220.
[27] 刘愚,李振国,魏佩珩,等.植物组织受伤后乙烯乙烷产生的时间进程——两种逆境乙烯的区分[J].植物生理学报,1982,8(1):81-89.Liu Y,Li Z G,Wei P H,et al.Time courses of ethylene and ethane production in plant tissues following mechanical wounding-distinction of two kinds of stress ethylene[J].Acta Phytophysiologia Sinica,1982,8(1):81-89.
[28] Saltveit M E Jr.,Dilley D R.Rapidly induced wound ethylene from excised segments of etiolated Pisum sativum L.,cv.Alaska:Ⅲ.Induction and transmission of the response[J].Plant Physiology,1978,62(5):710-712.