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Bulletin of Botanical Research ›› 2019, Vol. 39 ›› Issue (5): 770-778.doi: 10.7525/j.issn.1673-5102.2019.05.016

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Genetic Identification and Relationship Analysis of Main Southern-type Poplar Clones with EST-SSR

YUAN Jia-Qiu, TIAN Ye, FU Xiang-Xiang   

  1. Co-Innovation Center for the Sustainable Forestry in Southern China, College of forestry, Nanjing forestry university, Nanjing 210037
  • Received:2019-01-30 Online:2019-09-05 Published:2019-07-16
  • Supported by:
    The National Key Technology R&D Program(2015BAD09B02);The National Natural Science Foundation of China(31370618);The National Key Research and Development Program of China(2016YFD0600402)

Abstract: Twelve main southern-type poplar clones were selected to assess genetic polymorphism based on EST-SSR markers and to establish fingerprint for clone identification in the main planting region. The results could provide information and tools for the effective and protective extension of superior clones in this area. Thirty pairs of EST-SSR primers were obtained with stepwise approaches including EST sequences analysis from NCBI database by EST-trimmer, SSR loci screening by MISA, and primers design using primer 3. The obtained EST-SSR primers were then applied to PCR amplification for the 12 poplar clones. Eighteen pairs of polymorphic primers were selected and 88 alleles were obtained by sequence amplification of 12 poplar clones. The polymorphism ratio of the locus was 70.5%, the average number of the observed alleles was 2.1463, and the average shannon's diversity index was 0.529 7. The 12 clones could be effectively identified based on a combination of 5 pairs of SSR primers, which were EU147, EU43, EU11, EU164, and EU81. The genetic fingerprint map of the 12 clones was also constructed successfully using the same SSR primers. The genetic similarity coefficient of the 12 clones ranged from 0.602 0-0.904 0, with a mean value of 0.769 1. All the clones showed close genetic distance, which revealed their genetic background of the same origination. In a word, EST-SSR marker is an effective tool for the identification of the southern type poplar clones.

Key words: southern type poplar clone, EST-SSR marker, clone identification, polymorphism

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