Genetic Identification and Relationship Analysis of Main Southern-type Poplar Clones with EST-SSR

doi:10.7525/j.issn.1673-5102.2019.05.016

Abstract

Abstract: Twelve main southern-type poplar clones were selected to assess genetic polymorphism based on EST-SSR markers and to establish fingerprint for clone identification in the main planting region. The results could provide information and tools for the effective and protective extension of superior clones in this area. Thirty pairs of EST-SSR primers were obtained with stepwise approaches including EST sequences analysis from NCBI database by EST-trimmer, SSR loci screening by MISA, and primers design using primer 3. The obtained EST-SSR primers were then applied to PCR amplification for the 12 poplar clones. Eighteen pairs of polymorphic primers were selected and 88 alleles were obtained by sequence amplification of 12 poplar clones. The polymorphism ratio of the locus was 70.5%, the average number of the observed alleles was 2.1463, and the average shannon's diversity index was 0.529 7. The 12 clones could be effectively identified based on a combination of 5 pairs of SSR primers, which were EU147, EU43, EU11, EU164, and EU81. The genetic fingerprint map of the 12 clones was also constructed successfully using the same SSR primers. The genetic similarity coefficient of the 12 clones ranged from 0.602 0-0.904 0, with a mean value of 0.769 1. All the clones showed close genetic distance, which revealed their genetic background of the same origination. In a word, EST-SSR marker is an effective tool for the identification of the southern type poplar clones.

Key words: southern type poplar clone, EST-SSR marker, clone identification, polymorphism

CLC Number:

S722.3

YUAN Jia-Qiu, TIAN Ye, FU Xiang-Xiang. Genetic Identification and Relationship Analysis of Main Southern-type Poplar Clones with EST-SSR[J]. Bulletin of Botanical Research, 2019, 39(5): 770-778.

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