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Bulletin of Botanical Research ›› 2014, Vol. 34 ›› Issue (6): 798-802.doi: 10.7525/j.issn.1673-5102.2014.06.014

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Identification of Sex Related SRAP Molecular Marker in Humulus scandens


  1. College of Life Science,Henan Normal University,Xinxiang 453007
  • Received:2014-04-22 Online:2014-11-20 Published:2015-01-06
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Abstract: Humulus scandens is a model material for studying the molecular mechamisms of sex differentiation in dioecious plants. We used SRAP (sequence related amplified polymorphism) to screen male or female specific fragments in H.scandensby using male and female gene pool as template. Fifty pairs of primers showed a good amplification pattern after screening 256 pairs of primers. Polyacrylamide gel electrophoresis amplification patterns of SRAP showed that 671 bands were amplified using 50 primer combinations, of which 247 bands were polymorphic and the polymorphic ratio is 36.81%. With five single male plants and five single female plants as materials, we validated the amplification patterns of primer combinations me15+em11, me14+em16, me13+em12 and me7+em10 again by agarose gel electrophoresis. Although the amplification patterns were more or less different between polyacrylamide gel electrophoresis and agarose gel electrophoresis, male or female specific bands were relatively stable.If cloning, sequencing and chromosomal locating the obtained sex-related DNA sequences on chromosome of H.scandens by using fluorescence in situ hybridization technique, it will be contributed to studying the molecular mechanisms of sex chromosome evolution in H.scandens.

Key words: Humulus scandens Siebold &, Zucc;dioecism;sequence related amplified polymorphism

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