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Bulletin of Botanical Research ›› 2019, Vol. 39 ›› Issue (3): 358-364.doi: 10.7525/j.issn.1673-5102.2019.03.005

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Key Technology of Micropropagation of Osmanthus fragrans var. thunbergii

YE Tian-Yi, ZHOU Xin, HUANG Yan, WANG Xiao-Hong   

  1. Harbin Research Institute of Forestry Machinery, State Forestry Administration, Harbin 150086
  • Received:2018-08-09 Online:2019-05-05 Published:2019-05-11
  • Supported by:
    Special fund project of basic scientific research operation of central public welfare research institutes(CAFYBB2017MA026)

Abstract: We studied the effects of different rooting media, pre-treatment of seedling cultivation, transplanting matrix and leaf surface fertilization on the seedling cultivation of micro-propagation of Osmanthus fragrans to provide technical support for the rapid propagation of strong seedlings of target varieties, and the key technology of rapid propagation of O.fragrans. The optimal combination of rooting rate and rooting number of micro-propagated O.fragrans seedlings was A3B2C3, the rooting rate and average rooting number of B5 basic medium+2 mg·L-1 NAA+0.10 mg·L-1 6-BA were 91.11% and 3.83 pieces/plant, respectively. A3B2C3 is the optimal combination for seedling refining, that is, when the nursery soil:perlite:humus=2:3:7 was used as the seedling refining matrix, the survival rate and growth rate of micropropagation seedlings of O.fragrans reached 82.2% and 42.2%, respectively.

Key words: Osmanthus fragrans, micropropagatio, hardening, transplantings

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