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Bulletin of Botanical Research ›› 2017, Vol. 37 ›› Issue (3): 474-480.doi: 10.7525/j.issn.1673-5102.2017.03.020

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Process Optimization of Ultrasonic Assisted Deep Eutectic Solvents(DESs) Extraction of Polyphenols from Hippophae rhamnoides Seed Meal

NI Yu-Jiao1, ZHAO Chun-Jian1, LI Chun-Ying1, LI Guo-Chun2, WANG Kai-Ting1, ZHANG Yu-Kun1   

  1. 1. Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040;
    2. Heilongjiang Province Forestry Survey and Planning Institute, Harbin 150040
  • Received:2017-02-27 Online:2017-05-15 Published:2017-06-03
  • Supported by:
    The Fundamental Research Funds for the Central Universities(2572015EA04)

Abstract: A range of deep eutectic solvents(DESs) as the solvent were used for extraction polyphenols with ultrasonic-assisted extraction methods from sea buckthorn. According to the single-parameter study, experiment factors(ultrasonic power、ultrasonic time、ultrasonic temperature) remarkably affected the yield of polyphenols were selected and Box-Behnken experimental design was investigated to be optimized by response surface methodology. The results indicated that with 30% water in Choline chloride-Oxalic acid as the suitable solvent and liquid to material ratio of 14:1, the optimal conditions of ultrasonic extraction were as follows:ultrasonic power of 420 W, ultrasonic time of 56 min, and ultrasonic temperature of 44℃. Under these conditions, the yield of polyphenols was(3.31±0.08)%. Comparison experiment indicated that the extraction efficiency of polyphenols from sea buckthorn seed meal by DES was superior to the traditional solvent. Compared with the heat refluxing extraction method, DESs extraction method was a extraction technology with high efficiency.

Key words: deep eutectic solvents(DESs), ultrasonic-assisted extraction, sea buckthorn seed meal, polyphenol

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