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Bulletin of Botanical Research ›› 2016, Vol. 36 ›› Issue (3): 354-359.doi: 10.7525/j.issn.1673-5102.2016.03.006

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Molecular and Cytological analysis of Meiosis in Autotetraploid Arabidopsis thaliana

LI Yun-Ling, TIAN Bao-Ming, YANG Yan, CUI Ming-Zhu, HAO Zhi-Da, WEI Fang   

  1. School of Life Sciences, Zhengzhou University, Zhengzhou 450001
  • Received:2015-10-23 Online:2016-05-15 Published:2016-05-20

Abstract: The diploid Arabidopsis thaliana(Columbia-0, 2n=10) was used to obtain autotetraploid plants(2n=20) after 0.2% colchicine treatment and cytological identification. Meiotic chromosome behaviors were comparatively observed in diploid and autotetraploid A.thaliana, and the meiosis-related genes were relatively quantified by Real-time PCR. The autotetraploid A.thaliana produced relatively larger stoma and fruits, but the stomatal density and the seed-set were significantly reduced. Some chromosomal configurations were also observed during meiosis involving the trivalents, univalents, bivalents and quadrivalents in autotetraploid plants. The expression of several meiosis-related genes including ASY1, DMC1, MRE11 and SPO11-1 were up-regulated in autotetraploids. Therefore, as autopolyploidization proceeds, plants may adapt themselves into environment by accompanying with phenotype changes owing to the altered chromosomal behaviors and gene expressions.

Key words: Arabidopsis thaliana, diploid, autotetraploid, meiosis, chromosome

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