Variation Analysis of Growth Traits of Transgenic Populus simonii×P.nigra Clones Carrying TaLEA Gene

doi:10.7525/j.issn.1673-5102.2015.04.011

Abstract

Abstract: We used 11 Populus simonii×P.nigra clones including ten transgenic poplar clones and one control clone in six-year old to study 15 traits including growth traits, shape of stem, leaf traits, and lenticel traits. By ANOVA analysis, there was significant difference in all the traits among 11 clones(P＜0.01). The coefficients of phenotypic variation(PCV) of different traits ranged in 1.92%-39.98%, the coefficients of genetic variation(GCV) were closed to PCV and repeatability(R) ranged in 0.621-0.987, which indicated that the TaLEA gene transfer can influence the plant growth. but the impact were strong controlled by genetic factor. By phenotypic correlation analysis, there were varying degree correlations between growth traits, stem traits, leaf traits and lenticel traits, which indicated that all the traits combined action on plant growth and development. When the selection rate was 10%, XL-1 and XL-9 were selected as the best clones. The average height and diameter at breast height of these two clones were higher than those of CK(average of all the clones) by 16.55%(13.38%) and 15.38%(19.46%), and the genetic gain of these clones were 12.18% and 17.42% in height and diameter at breast height, respectively.

Key words: Populus simonii×P.nigra, TaLEA gene, correlation analysis, genetic gain

CLC Number:

LIU Dian-Kun1；LIU Meng-Ran1；LI Zhi-Xin1；WANG Guang-Yu2；LI Ying1；ZHENG Mi1；LIU Gui-Feng1；ZHAO Xi-Yang1*. Variation Analysis of Growth Traits of Transgenic Populus simonii×P.nigra Clones Carrying TaLEA Gene[J]. Bulletin of Botanical Research, 2015, 35(4): 540-546.

References

1.徐纬英.杨树［M］.哈尔滨：黑龙江人民出版社，1988.

2.赵曦阳.白杨杂交试验与杂种无性系多性状综合评价［D］.北京：北京林业大学,2010.

3.江泽慧，范少辉，冯慧想，等.华北沙地小黑杨人工林生物量及其分配规律［J］.林业科学，2007，43（11）：15-20.

4.范少辉，刘广路，张群，等.华北沙地小黑杨生物量及其与树冠关系的研究［J］.林业科学研究，2010，23（1）：71-76.

5.姜鹏，崔丽红，秦召文，等.小黑杨胸径和树高的生长动态［J］.西北林学院学报，2013，28（6）：129-133.

6.宋杨，张潆心，郭娜，等.低温胁迫后不同光强对小黑杨幼苗叶片叶绿素荧光和能量分配的影响［J］.安徽农业科学，2013，41（10）：4421-4423.

7.王雷，周博如，吴丽丽，等.小黑杨环锌指蛋白基因的克隆与表达分析［J］.植物生理学通讯，2009，45（12）：1160-1166.

8.王遂，刘梦然，黄海娇，等.转TaLEA基因小黑杨抗寒株系的筛选［J］.东北林业大学学报，2011，39（9）：5-7，16.

9.李志新，赵曦阳，杨成君，等.转TaLEA基因小黑杨株系变异及生长稳定性分析［J］.北京林业大学学报，2013，35（2）：57-62.

10.Velten J,Oliver M J.Tr288,a rehydrin with a dehydrin twist［J］.Plant Mol Biol,2001,45(6):713-722.

11.Campos F,Zamudio F,Covarrubias A.Two different late embryogenesis abundant proteins from Arabidopsis thaliana contain specific domains that inhibit E.coli growth［J］.Biochem Biophys Res Commun,2006,342:406-413.

12.Singh S,Cornilescu C C,Tyler R C,et al.Solution structure of a late embryogenesis abundant protein (LEA14) from Arabidopsis thaliana,a cellular stress-related protein［J］.Protein Sci,2005,14:2601-2609.

13.Gal T Z,Glazer I,Koltai H.An LEA group 3 family member is involved in survival of C.elegans during exposure to stress［J］.FEBS Lett,2004,577:21-26.

14.Hundermark M,Hincha D K.LEA(Late Embryogenesis Abundant) proteins and their encoding genes in Arabidopsis thaliana［J］.BMC Genomics,2008,9:118doi:10.1186/1471-2164-9-118.

15.白爽.转Lea基因小黑杨花粉植株抗旱、耐盐性分析［D］.哈尔滨:东北林业大学，2007:37-38.

16.赵曦阳，马开峰，沈应柏，等.白杨派杂种无性系植株早期性状变异与选择研究［J］.北京林业大学学报，2012，34（2）：45-51.

17.丘进清.闽北柳桉种源/家系的试验研究［J］.中南林业科技大学学报，2007，27（3）：33-36.

18.黄德龙.柳桉家系适应性试验与遗传变异分析［J］.山地农业生物学报，2008，27（3）：207-212.

19.时立文.SPSS 19.0统计分析从入门到精通［M］.北京：清华大学出版社，2012:28-29.

20.续九如.林木数量遗传学［M］.北京：中国林业出版社，2006:31,55.

21.解孝满，李景涛，赵合娥.柳树无性系苗期遗传测定与选择［J］.江苏林业科技，2008，35（3）：6-14.

22.朱之悌.林木遗传学基础［M］.北京：中国林业出版社，1989:190-191.

23.Su X H,Chu Y G,Li H,et al.Expression of multiple resistance genes enhances tolerance to environmental stressors in transgenic poplar(Populus×euramericana ‘Guariento’)［J］.PLoS ONE,2011,6(9):e24614.

24.刘梦然，边秀艳，麻文俊，等.转TaLEA基因小黑杨无性系苗期生长性状Logistic模型构建［J］.植物研究，2014，34（4）：485-491.

25.Zhao X Y,Hou W,Zheng H Q,et al.Analyses of genotypic variation in white poplar clones at four sites in China［J］.Silvae Genetica,2014,62,4-5:187-195.

26.Pliura A,Zhang S Y,Mackay J,et al.Genotypic variation in wood density and growth traits of poplar hybrids at four clonal trials［J］.Forest ecology and management,2007,238:92-106.

27.Maniee M,Kahrizi D,Mohammadi H.Genetic variability of some morphophysiological traits in durum wheat(Triticum turgidum var. durum)［J］.Journal of Applied Sciences,2009,9:1383-1387.

28.Kien N D,Jansson C,Harwood C,et al.Genetic variation in wood basic density and Pilodyn penetration and their relationships with growth, stem straightness and branch size for Eucalyptus urophylla S.T.Blake in Northern Vietnam［J］.NZ J For Sci,2008,38:160-175.

29.李善文，乔玉玲，姜岳忠，等.黑杨派无性系多性状相关分析［J］.山东林业科技，2006（2）：11-13.

30.董玉峰，姜岳忠，张明哲，等.不同杨树品种的分枝及与生长和干形的关联［J］.中南林业科技大学学报，2014，34（2）：34-38.

31.李善文，姜岳忠，吴德军，等.杨树无性系多性状相关分析［J］.山东林业科技，2006（1）：18-20.

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