Expression of A Floral Homeotic Gene PrseSTK from Prunus lannesiana in Single and Double Flower

doi:10.7525/j.issn.1673-5102.2015.04.010

Bulletin of Botanical Research ›› 2015, Vol. 35 ›› Issue (4): 535-539.doi: 10.7525/j.issn.1673-5102.2015.04.010

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Expression of A Floral Homeotic Gene PrseSTK from Prunus lannesiana in Single and Double Flower

LIU Zhi-Xiong^1,2；LI Feng-Lan²

1.College of Horticulture and Gardening,Yangtze University,Jingzhou　434025；
2.College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing　100083

Online:2015-07-20 Published:2016-03-09
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Abstract

Abstract: To find out genes involved in the floral organogenesis of Prunus lannesiana, we cloned cDNAs of one MADS-box genes, PrseSTK with GenBank accession No.GU332504 by using homologous cloning. The full length of PrseSTK contained an open reading frame(ORF) of 669 bp and coding for a polypeptide of 222 amino acid residues. By sequence comparison and phylogenetic analysis, PrseSTK was highly homologous to protein STK in Arabidopsis, and belonged to class D. However, by semiquantitative RT-PCR, PrseSTK was highly expressed in stamen and gynoecium of single-flowered P.lannesiana cultivar ‘Makino’, and in sepal, stamen and leaf-like gynoecium of doubleflowered cultivar ‘Albo-rosea’, which was significantly different from the expression pattern of STK in Arabidopsis. The different expression of PrseSTK in double flower showed ectopic ovules formation would result from PrseSTK expression in the sepal.

Key words: Prunus lannesiana, flower development, double flower, SEEDSTICK

CLC Number:

LIU Zhi-Xiong1,2；LI Feng-Lan2. Expression of A Floral Homeotic Gene PrseSTK from Prunus lannesiana in Single and Double Flower[J]. Bulletin of Botanical Research, 2015, 35(4): 535-539.

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Expression of A Floral Homeotic Gene PrseSTK from Prunus lannesiana in Single and Double Flower

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