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Bulletin of Botanical Research ›› 2011, Vol. 31 ›› Issue (4): 499-502.doi: 10.7525/j.issn.1673-5102.2011.04.019

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Comparison of the Determination Methods for Total Flavonoids in Pigeon Pea Leaves

ZHAO En-Ze;JIN Shi;WEI Zuo-Fu;WEI Wei;FU Yu-Jie;*   

  1. 1.Key Laboratory of Forest Plant Ecology of Northeast Forestry University,Ministry of Education,Harbin　150040；2.Engineering Research Center of Forest Biopreparation,Ministry of Education,Northeast Forestry University,Harbin　150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-07-20 Published:2011-07-20
  • Contact: FU Yu-Jie
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Abstract: AlCl-3, boric acid-citric acid-and Al(NO)3-based colorimetric assays were compared to determine the total flavonoids in pigeon pea leaves. The results indicated that Al(NO)3 colorimetric assay was the most appropriate for the determination of total flavonoids in pigeon pea leaves. The stability, precision and repeatability were very good, with RSD being 2.1%(n=3), 0.9%(n=3) and 2.3%(n=5), respectively. The content of total flavonoids in pigeon pea leaves was 15.65 mg·g-1 DW. The method is appropriate for the quantitative determination of total flavonoids in pigeon pea leaves and their preparations. It can be referenced for the quality control of pigeon pea leaves.

Key words: pigeon pea leaves, total flavonoids, colorimetric assay

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