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Bulletin of Botanical Research ›› 2011, Vol. 31 ›› Issue (3): 367-370.doi: 10.7525/j.issn.1673-5102.2011.03.021

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The Extraction Process of Biochanin A from Dalbergia odorifera T. Chen Leaves

WANG Fang;ZHANG Dong-Yang;MA Fei-Yue;GAI Qing-Yan;JIAO Jiao;FU Yu-Jie;*   

  1. 1.Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Haerbin 150040；2.Engineering Research Center of Forest Bioprepration,Ministry of Education,Northeast University,Haerbin 150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-05-20 Published:2011-05-20
  • Contact: FU Yu-Jie
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Abstract: The preliminary experiments and a three-variable, three-level Box-Behnken design were performed to study the microwave-assisted enzymatic extraction of biochanin A from Dalbergia odorifera T. Chen leaves. The optimum parameters were: extraction time 15 min, irradiation power 300 W, irradiation temperature 34℃, pH 5.2, amount of cellulase 3.5 mg·g-1. Under the optimal conditions, the average extraction yield of biochanin A was 1.579 mg·g-1.

Key words: Dalbergia odorifera T. Chen leaves, Biochanin A, microwave-assisted enzymatic extraction, Box-Behnken design

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