Abstract: The content of polysaccharide from leaves, branches and seeds of endangered plants Elaeagnus mollis Diels was determined by the phenol-sulfuric acid method under 490 nm. The oxygen anion radical and hydroxyl radical scavenging activity of polysaccharide from three parts of E.mollis was studied by the pyrogallol self-oxidation method and spectrophotometry with safranine O—Mn²⁺—H₂O₂ system. The results showed that the average levels of polysaccharide from leaves, branches and seeds of E.mollis were 69.42, 23.15 and 56.47 mg·g^-1, respectively. The polysaccharide of three parts showed antioxidant activities in removing superoxide free radicals and hydroxyl radical. The scavenging activity on superoxide free radicals was Vc>seeds>branches>leaves, removing hydroxyl radical activity was leaves>seeds>Vc>branches. The research results showed that the leaves and seeds of E.mollis could be the raw material as natural antioxidant.

Key words: Elaeagnus mollis Diel, plant polysaccharide, antioxidant activity