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Bulletin of Botanical Research ›› 2011, Vol. 31 ›› Issue (1): 113-116.doi: 10.7525/j.issn.1673-5102.2011.01.021

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GC-MS Analysis and in Vitro Antitumor Activity of the Extract from Typhonium giganteum Engl using Supercritical Fluid CO2

LI Qing-Yong;WANG Chun-Cheng;SONG Zhen;QU Zhen-Huan;JIANG Chun-Fei;QIU Wei   

  1. Key Laboratory of Forest Plant Ecology of Northeast Forestry University,Ministry of Education,Harbin　150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-01-20 Published:2011-01-20
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Abstract: In order to explore the antitumor active components of Typhonium giganteum Engl, the powder of T.giganteum Engl tubers was extracted using supercritical fluid CO2-80% ethanol, and the extract was identified by gas chromatography-mass spectrometry(GC-MS) technology. The in vitro antitumor effects of the extract was evaluated by MTT colorimetric method. Thirty-seven compounds were identified from the extract by GC-MS analysis. Four major components are as follows: β-Sitosterol, accounting for 40.22%; Campesterol, accounting for 18.45%; n-Hexadecanoic acid, accounting for 9.52%; 9,12-Octadecadienoic acid (Z,Z)-, accounting for 8.15%. MTT experiments indicated that the extract showed significant inhibition on the colon cancer HCT-8, ovarian cancer HO-8910, gastric cancer SGC-7901, and liver cancer SMMC-7721 cells. Liver cancer SMMC-7721 cells were the most sensitive to the extract. The extract of T.giganteum Engl tubers by supercritical fluid CO2 showed remarkable anti-tumor effect. Further research on this extract is necessary to find its active substances for reasonable development and utilization of T.giganteum Engl.

Key words: Typhonium giganteum Engl, supercritical fluid CO2 extraction, GC-MS analysis, antitumor activity

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