Welcome to Bulletin of Botanical Research! Today is Share:

Bulletin of Botanical Research ›› 2010, Vol. 30 ›› Issue (1): 32-36.doi: 10.7525/j.issn.1673-5102.2010.01.008

Previous Articles     Next Articles

Effects of He-Ne Laser and Enhanced UV-B Radiation on Chloroplast Pigments in Wheat Seedlings

GAO Li-Mei;LI Yong-Feng;HAN Rong*   

  1. College of Life Science,Shanxi Normal University,Linfen 041004
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-01-20 Published:2010-01-20
  • Contact: HAN Rong
  • Supported by:

Abstract: The seedlings of wheat were exposed to He-Ne laser(5 mW·mm-2) irradiation and enhanced UV-B(10.08 kJ·m-2d-1) radiation. Then the content changes of chloroplast pigments and the composition of pigment-proteins were measured in order to test the mitigative effect of He-Ne laser irradiation on injury by enhanced UV-B radiation. After treating wheat seedlings for 4 days circularly, chloroplast pigments were extracted respectively by 90% ethanol and 80% acetone. The contents of chloroplast pigments were detected with spectrophotometer or paper chromatography. The composition of pigmentproteins were detected with column chromatography. It suggested that the content of chloroplast pigments decreased 17.76% approximately in wheat seedlings by enhanced UV-B radiation. The binding degree of Chl-Pro is weakened. The composition of pigment-proteins also differed from the control group. Straps of D1 and D2 proteins lost. However, the content of chloroplast pigments increased about 10.64% exposed to He-Ne laser irradiation, but decreased 8.12% comparing with the control. The binding degree of Chl-Pro is higher than the B group. At the same time, the composition of pigmentproteins was similar with the control group. So the lower dose of He-Ne laser irradiation can partly repair the radiation injury on chloroplast pigments and pigment-proteins in wheat seedlings after enhanced UV-B radiation.

Key words: He-Ne laser, ultraviolet-B radiation, chloroplast pigment, wheat

CLC Number: