Abstract: Using winter bud of Davidia involucrate as explant, we studied its tissue culture and plant regeneration. The results indicated: the best medium for the clump shoot induction directly from D. involucrata winter bud was WPM+NAA 0.2 mg·L^-1+6-BA 3.0 mg·L^-1+AC 2.0 g·L^-1; the appropriate medium for shoot segment multiplying of D. involucrate bud was WPM+NAA 0.05 mg·L^-1+6-BA 1.0 mg·L^-1+GA₃ 2.0 mg·L^-1+AC 2.0 g·L^-1; the medium for rooting culture was White+IBA3.0 mg·L^-1+6-BA 1.0 mg·L^-1+AC 2.0 g·L^-1, under these conditions, the roots were well developed and the plants were healthy; finally, about 80% of these seedlings could survival after transplantation.

Key words: Davidia involucrate Baill, winter bud, clump shoot, shoot segment multiplying, plant regeneration