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Bulletin of Botanical Research ›› 2008, Vol. 28 ›› Issue (6): 770-774.doi: 10.7525/j.issn.1673-5102.2008.06.027

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Analysis of the Volatile Constituents from Seeds of Polygonum orientale by Gas Chromatography-Mass Spectrometry Coupled with Solid-phase Microextraction

CHEN Yan;XUE Xiao-Juan;ZHU Hong*   

  1. (1.School of Chemistry and Environment,South China Normal University,Guangzhou　510006) (2.Institute of Subtropical Agriculture,Chinese Academy of Science,Changsha　410125)
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-11-20 Published:2008-11-20
  • Contact: ZHU Hong
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Abstract: Effect of sample temperature and adsorption time on the SPME(solid phase microextraction) treatment of seeds of Polygonum orientale L. were investigated. The optimal extraction conditions were absorption at 60℃ for 60 min and desorption at 250℃ for 5 min. Samples of seeds of P. orientale L. were analyzed by GC-MS coupled with SPME. Through comparison with mass spectra database，forty-tree components were identified，covering 76.73% of the total peak areas in the GC-MS total ion current chromatography. The characteristic components were Thujopsene(6.99%),Caryophyllene5.59%),2,5,5,8a-Tetramethyl-6,7,8,8a-tetrahydro-5H-naphthalen-1-one(5.52%),α-Caryophyllene(4.29%),1,2,4a,5,6,8a-hexahydro-4,7-dimethyl-1-(1-methylethyl)-Naphthalene(4.04%),Caryophylleneoxide(3.60%),α-Cyperone(3.54%),4,5,5a,6,6a,6b-hexahydro-4,4,6b-trimethyl-2-(1methylethenyl)-2H-Cyclopropa-benzofura n(3.54%),Geranylacetone(3.48%).

Key words: seeds of Polygonum orientale L., volatile constituents, solid phase microextraction, gas chromatography-mass spectrometry(GC-MS)

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