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Bulletin of Botanical Research ›› 2004, Vol. 24 ›› Issue (3): 347-350.

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A new efficient method for rapid in-vitro shoot regeneration on leaf explants of Poplar 741

WU Shuang-Xiu1,2, WU Madeline2, ZU Yuan-Gang1   

  1. 1. Key Laboratory of Forest Plant Ecology of the Ministry of Education, Northeast Forestry University, Harbin 150040;
    2. Department of Biology, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, SAR, People's Republic of China
  • Received:2003-12-01 Online:2004-09-15 Published:2016-06-14
  • Supported by:
    This research has been supported by the grant of Project 973(G19990160) and part ly supported by g rant HKUS T 6117/01M from the Research Grant Council of Hong Kong.

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Abstract: In the experiments of Agrobacterium tumefaciens-mediated transformation of the hybrid poplar 741,Populus alba(P.davidiana×P.simonii)×P.tomentosa,we developed a rapid and efficient method of shoot regeneration from the leaf explants in-vitro on Murashige and Skoog (MS) medium.The leaf explant segments was first cultured on medium I (MS medium supplemented with 0.5 mg/L of BA and 1.0 mg/L of 2,4-D) for 2~3 days and then transferred to medium SH (MS medium containing 2.0 mg/L of BA and 0.1 mg/L of NAA) to culture for 10 days.86.7% of leaf segments produced shoots after the leaf segments were transferred from medium SH to medium II (MS medium with 0.5 mg/L of BA) for 1 week.The average shooting number was 40~50 per 1 cm×1 cm of leaf segment.However,if the leaf explants incubated on medium I longer than 5 days,they produced more roots instead of shoots.

Key words: Poplar 741, Populus alba(P. davidiana×, P. simonii)×, P. tomentosa, shooting, regeneration