Abstract: Seeds of Syringa reticulata var.mandshurica stratified for 10,20 and 40 days were in vitro cultured.Before incubation,three different treatments of seeds were conducted:intact,middlecut,two ends-cut.The results showed that germination percentage of cut seed was enhanced,middle-cut was the best and achieved maximum germination after 10 days culture;germination of seeds treated 40 days was better than that of the seeds with other two treatments.In vitro germination of seeds with 40 days stratification was best on MS medium with 5 mg/L BA or BA 5 mg/L BA+0.1 mg/L IBA.

Key words: Syringa reticulata var .mandshurica, in vi tro culture, seed germination percentage