Abstract: In this paper, a thorough study on hypocotyl regeneration system of Betula platyphylla was made. We discussed effects of different basal culture media, hormones, culture conditions and explants'physiological ages on different culture stages. Through induction, differentiation and rooting stages, regenerative plants were gotten. Optimum basal culture media, hormone combinations, culture conditions and hypocotyl explants'physiological ages were put forward. These offer a good base for B. platyphylla genetic transformation.

Key words: Betula platyphylla, Hypocotyl, Regeneration system