REGENERATION OF BETULA PLATYPHYLLA BY LEAF EXPLANT

Abstract

Abstract: The regeneration system of birch Betula platyphylla by leaf explant was established. Different kinds of hormones (BA, KT, 2, 4-D, NAA, GA₃), basal media (MS, WP), explants orientation were experimented. The leaf explant was placed on medium with the adaxial face downwards. After three weeks, adventitious buds sprouted from the edge of leaf explants. The percentage of the adventitious shoot induction is 64%. The number of adventitious shoots incuced per leaf is 6.The regeneration system can be potentially used for genetic transformation of white birch.

Key words: White Birch, Regeneration, Leaf explant

SHI Fu-chen, KONG Jin, WU Shuang-xiu, NIE Ming-zhu, ZU Yuan-gang. REGENERATION OF BETULA PLATYPHYLLA BY LEAF EXPLANT[J]. Bulletin of Botanical Research, 2001, 21(4): 611-614.

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REGENERATION OF BETULA PLATYPHYLLA BY LEAF EXPLANT

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