Bulletin of Botanical Research ›› 2000, Vol. 20 ›› Issue (4): 420-426.
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HUANG Yong-Hong1, YOU Feng-Li1, KONG Ruo-Dong1, WANG Fu-De1, WANG Qing-Yin2, FU Gui-Rong2, HUANG Yong-Fen2
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Abstract: By means of the pollen tube pathway, the Ti plasmid which carrying the SAUR-ipt gene was transferred into Trigonella. Foonella. Foenum. graecum. We tested D1 generation plants with DNA southern blot hybridization and got five transformed plants. Analyzing the changes of morphology physiology and field characters of D3, D4 and D5 generation transformed plants, we found that the plants were dwarf slightly, amount of branch and double-legume increased, the shape of leaf became narrow, the content of chlorophyll and plant pastern increased in D4 generation, and solvable protein and ZRs increased in D5 generation. They are all reveal positive pertinence for the yield. Therefore, it is proved that SAUR-ipt gene already transferred into Trigonella. foonella. foenum. graecum.
Key words: Trigonella. foonella. foenum. graecum, Ti plasmid, ipt gene, pollen tube pathway
HUANG Yong-Hong, YOU Feng-Li, KONG Ruo-Dong, WANG Fu-De, WANG Qing-Yin, FU Gui-Rong, HUANG Yong-Fen. STUDY ON SAUR-IPT GENE TRANSFORMED TRIGONELLA. FOONELLA. FOENUM. GRAECUM[J]. Bulletin of Botanical Research, 2000, 20(4): 420-426.
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https://bbr.nefu.edu.cn/EN/Y2000/V20/I4/420
