EFFECTS OF PENICILLU M CITRINU M ELICITOR ON THE BIOSYNTHESIS OF TAXOL IN SUSPENSION CELL CULTURES OF TAXUS CHINENSIS

Bulletin of Botanical Research ›› 1998, Vol. 18 ›› Issue (1): 78-82.

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EFFECTS OF PENICILLU M CITRINU M ELICITOR ON THE BIOSYNTHESIS OF TAXOL IN SUSPENSION CELL CULTURES OF TAXUS CHINENSIS

Li Jia-ru¹, Liu Man-xi², Cao Meng-de³, Wang Jun-jian²

1. Institute of Hydrobiology, The Chinese Academy of Science, Wuhan 430072;
2. Bioengineering Department, Huazhong University of Science & Technology, Wuhan 430074;
3. Department of Basic Science Henan Medical University, Zhengzhou 450052

Received:1997-03-10 Online:1998-03-15 Published:2016-06-14

Abstract

Abstract: The Penicillum citrinum elicitor was extraxted after autoclaveing a 6~7-day old culture of P.citrinum which could promote taxol biosynthsis in suspension cell cultures of Taxus chinensis.The optimum elicitor cocentration was found to be 50μg carbohydrate/ml medium of T.chinensis cell cultures. T.chinensis cells showed strongest response to the elicitor during the growth phase(20d).The time of autoclaving(20~90min) did not affect the elicitor's activity.

Key words: Penicillum citrinum, Elicitor, Taxol, Cell culture, Taxus chinensis

Li Jia-ru, Liu Man-xi, Cao Meng-de, Wang Jun-jian. EFFECTS OF PENICILLU M CITRINU M ELICITOR ON THE BIOSYNTHESIS OF TAXOL IN SUSPENSION CELL CULTURES OF TAXUS CHINENSIS[J]. Bulletin of Botanical Research, 1998, 18(1): 78-82.

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EFFECTS OF PENICILLU M CITRINU M ELICITOR ON THE BIOSYNTHESIS OF TAXOL IN SUSPENSION CELL CULTURES OF TAXUS CHINENSIS

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