doi:10.7525/j.issn.1673-5102.2022.02.015

Abstract

Abstract:

In order to explore the eco-physiological mechanism of Populus euphratica Oliv adapting to the arid desert environment，P. euphratica Oliv. that the key species in the Tarim desert ecosystem was used as material. It was conducted to research the change of photosynthetic gas exchange， antioxidative enzyme activity and osmoregulation substances of P. euphratica grown at different groundwater depths（GWD）. The results showed that：（1） the diurnal courses of net photosynthetic rate（P_n）， transpiration rate（T_r） and stomatal conductance（G_s） of P. euphratica under different GWD conditions showed the single peak curves respectively， while the diurnal variation of intercellular CO₂ mole fraction（C_i） were the “V” curve， the valley value was significantly reduced at GWD=5.5 m（P<0.01） respectively. P_n and G_s changed synchronously， and the peak value appeared at 12：00， while the T_r peak value lagged 2 hours. The peak values of P_n in different GWD were significantly different（P<0.05）， while the peak values of T_r， G_s and C_i valley were significantly reduced in GWD=5.5 m（P<0.01） respectively. P_n， T_r， G_s， C_i， water-use efficiency（WUE） and light utilization efficiency（LUE） decreased with GWD increasing， and the daily mean value of G_s and LUE significantly decreased at GWD=5.5 m（P<0.05） respectively. However， there was no significant difference in the daily mean values of P_n， T_r， C_i and WUE under different GWD conditions（P>0.05） respectively；（2）The decline of P_n in P. euphratica from 12：00-16：00 was mainly restricted by stomatal factors， while the decline of P_n from 16：00-20：00 was mainly limited by non-stomatal factors；（3）The relationship between eco-physiological factors and P_n， T_r under different GWD conditions were analyzed with correlation， partial correlation and stepwise regression methods， it was found that G_s was the main factor affecting P_n and T_r of P. euphratica respectively. The correlation degree between P_n and photosynthetically active radiation（PAR）， T_r and G_s were enhanced respectively with GWD increasing， indicating that GWD directly regulated the air-water exchange；（4）The malondialdehyde content（MDA）， superoxide dismutase activity（SOD）， peroxidase activity（POD） and free proline content（Pro） were increased respectively with GWD increasing， but the content of soluble protein（SP）， soluble sugar（SS） were both decreased， which indicated that the cell membrane permeability and photosynthetic carbon assimilation were inhibited respectively with GWD increasing. P. euphratica enhanced the protective enzyme activity（POD， SOD） and osmoregulation content（Pro） to cooperate against drought stress caused by GWD increasing， and ensured to maintain the basic normal physiological activities. This was the eco-physiological strategy for P. euphratica to adapt arid desert environment.

Key words: Populus euphratica Oliv., groundwater depth, gas exchange, osmoregulation substances, protective enzyme activity

CLC Number:

S792.11

Yaqi Dai, Yanping Liu, Lu Han, Haizhen Wang. [J]. Bulletin of Botanical Research, 2022, 42(2): 299-308.

Figures/Tables 8

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Table 2

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References 30

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样地 Plot	地下水埋深 GWD /m	土壤质量含水量 Soil moisture /%
样地 Plot	地下水埋深 GWD /m	0~20 cm	20~40 cm	40~60 cm	60~80 cm	80~100 cm	100~120 cm
Ⅰ	2.0	16.78	21.82	23.46	26.85	28.55	29.61
Ⅱ	3.6	1.58	2.25	2.73	3.31	4.40	9.52
Ⅲ	5.5	0.21	0.64	0.72	0.98	1.26	1.49

	样地 Plot	气孔导度 G_s /（mol·m^-2·s^-1）	胞间CO₂摩尔分数 C_i /（μmol·mol^-1）	气温 T_air /℃	光合有效辐射 PAR /（μmol·m^-2·s^-1）	大气相对湿度 RH /％	水汽压差 VPD /kPa	大气CO₂摩尔分数 C_a /（μmol·mol^-1）
净光合速率 P_n	Ⅰ	0.720 7^**	-0.781 8^**	0.273 8^*	0.821 0^**	0.096 5	-0.022 9	-0.176 0^*
	Ⅱ	0.698 0^**	-0.729 2^**	0.506 2^**	0.864 6^**	-0.579 7^**	0.423 3^**	-0.567 5^**
	Ⅲ	0.604 1^**	-0.780 6^**	0.181 5	0.901 5^**	0.113 3	0.065 7	-0.028 8
蒸腾速率 T_r	Ⅰ	0.551 8^**	-0.679 3^**	0.696 8^**	0.750 8^**	-0.442 5^**	0.407 9^**	-0.492 0^**
	Ⅱ	0.603 2^**	-0.691 5^**	0.772 6^**	0.806 4^**	-0.819 5^**	0.696 6^**	-0.637 4^**
	Ⅲ	0.621 3^**	-0.586 4^**	0.406 5^**	0.700 6^**	-0.203 1	0.275 5^**	-0.168 8

光合指标 Photosynthetic indexes	样地 Plot	气孔导度 G_s /（mol·m^-2·s^-1）	胞间CO₂摩尔分数 C_i /（μmol·mol^-1）	气温 T_air /℃	光合有效辐射 PAR /（μmol·m^-2·s^-1）	大气相对湿度 RH /％	水汽压差 VPD /kPa	大气CO₂摩尔分数 C_a /（μmol·mol^-1）
净光合速率 P_n	Ⅰ	0.779 0^**	-0.859 0^**	-0.148 5	-0.116 4	0.131 6	0.189 0^*	-0.018 0
	Ⅱ	0.762 2^**	-0.662 2^**	-0.342 9^**	0.038 0	-0.217 9^*	0.294 5^*	0.163 4
	Ⅲ	0.806 2^**	-0.805 6^**	0.478 9^**	-0.305 0^*	0.586 4^**	-0.152 1	0.069 9
蒸腾速率 T_r	Ⅰ	0.856 3^**	0.115 8	-0.262 8^*	0.397 3^**	-0.363 8^**	0.629 7^**	0.038 1
	Ⅱ	0.731 3^**	0.086 9	-0.072 1	0.058 8	-0.058 8	0.572 4^**	0.114 5
	Ⅲ	0.920 7^**	0.173 7	0.386 2^**	0.128 1	0.273 6^*	0.581 9^**	0.049 8

光合指标 Photosynthetic indexes	样地 Plot	最优回归方程 Optimal regression equation	R²
净光合速率 P_n	Ⅰ	P_n=64.482+30.186G_s-0.150C_i-0.683T_air+0.968VPD	0.977 0^**
	Ⅱ	P_n=46.811+29.954G_s-0.088C_i-0.937T_air-0.272RH+1.634VPD+0.037C_a	0.965 1^**
	Ⅲ	P_n=-7.435+43.106G_s-0.082C_i+0.676T_air-0.002PAR+0.567RH-0.613VPD	0.981 4^**
蒸腾速率 T_r	Ⅰ	T_r=9.786+21.322G_s+0.006C_i-0.401T_air+0.002PAR-0.157RH+2.523VPD	0.962 9^**
	Ⅱ	T_r=-16.512+21.963G_s-0.059T_air+2.868VPD+0.033C_a	0.956 8^**
	Ⅲ	T_r=-9.947+27.977G_s+0.182T_air+0.075RH+1.055VPD	0.975 5^**

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