Allelopathy and Its Mechanism of Three Natural Diterpenes

doi:10.7525/j.issn.1673-5102.2022.01.009

Abstract

Abstract:

To evaluate the allelopathic potential of three diterpenes， including wangzaozin A， leukamenin E and weisiensin B， Lolium perenne L. and Lacutuca sativa L. were taken as materials and the effects of three ent-kaurene diterpenes on the seed germination， growth， chlorophyll， membrane damage of tested plants were studied respectively. It was found that three diterpenes with different concentrations had a slightly decrease on germination of L. perenne L. and L. sativa L. seeds， but the germination time was delayed obviously； three diterpenes at higher concentrations had significant negative effects on the root length， seedling length， fresh weight and dry weight of plants respectively， and it showed clearly concentration-dependent trend； meanwhile， the chlorophyll a and chlorophyll b contents were significantly reduced but the malondialdehye（MDA） content and relative electricity conductivity were increased， however， the relative water content showed downward trends. The results showed that three diterpenoids inhibited the growth of tested plants by affecting its photosynthetic capacity， causing oxidative damage to the cell membrane and reducing the water retention of the leaves.

Key words: Lolium perenne L., Lacutuca sativa L., ent-kaurene diterpenes, allelopathy

CLC Number:

S543+.6

Zhaowei Huan, Qiaoming Ou, Lan Ding. Allelopathy and Its Mechanism of Three Natural Diterpenes[J]. Bulletin of Botanical Research, 2022, 42(1): 81-92.

Figures/Tables 9

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Table 1

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化合物种类 Compounds types	浓度 Concentration /（μmol·L^-1）	萌发率 Germination rate /%	萌发指数 Germination index	根长 Root length /cm	苗长 Seedling length /cm	鲜质量 Fresh weight /mg	干质量 Dry weight /mg
wangzaozin A	0	94.44±1.15	46.73±1.63	3.35±0.27	2.73±0.15	6.29±0.51	1.57±0.06
	5	90.00±2.65	39.93±3.19^**	2.86±0.18^**	2.56±0.12^**	6.11±0.48^*	1.23±0.21^**
	10	88.89±3.21	38.38±2.05^**	2.01±0.18^**	1.94±0.11^**	3.99±0.13^**	1.09±0.08^**
	25	85.56±1.53	35.51±1.90^**	1.14±0.06^**	1.48±0.05^**	2.40±0.14^**	0.83±0.07^**
leukamenin E	0	94.44±1.15	46.73±1.63	3.35±0.27	2.73±0.15	6.29±0.51	1.57±0.06
	5	95.56±0.58	47.52±1.10	3.05±0.31*	2.61±0.29	5.95±0.69	1.53±0.11
	10	90.00±3.46	39.32±3.37^**	2.78±0.42^**	1.96±0.08^**	5.22±0.23^**	1.44±0.04^**
	25	86.67±4.36	37.17±6.00^**	1.21±0.14^**	1.63±0.27^**	3.81±0.78^**	1.17±0.04^**
weisiensin B	0	94.44±1.15	46.73±1.63	3.35±0.27	2.73±0.15	6.29±0.51	1.57±0.06
	5	92.22±3.21	40.79±5.19	3.07±0.06^**	2.46±0.17^**	6.10±0.19	1.55±0.03
	10	87.78±2.52	37.63±3.46^*	2.74±0.15^**	2.12±0.08^**	5.73±0.40^**	1.50±0.21
	25	88.89±2.52	38.54±3.66^*	1.24±0.22^**	1.34±0.08^**	4.21±0.21^**	1.17±0.14^**

化合物种类 Compounds types	浓度 Concentration /（μmol·L^-1）	萌发率 Germination rate /%	萌发指数 Germination index	根长 Root length /cm	苗长 Seedling length /cm	鲜质量 Fresh weight /mg	干质量 Dry weight /mg
wangzaozin A	0	91.11±2.52	38.46±2.25	3.67±0.15	2.48±0.11	14.53±0.24	1.19±0.07
	2	86.67±1.73	32.43±1.78*	3.77±0.41	2.74±0.14^**	13.25±0.39^**	1.26±0.10^*
	4	85.56±3.06	31.06±4.07*	2.96±0.28^**	2.13±0.20^**	12.05±1.29^**	1.06±0.10^**
	8	80.00±1.73	26.82±1.89^**	1.17±0.31^**	0.98±0.13^**	8.49±1.45^**	0.96±0.088^**
leukamenin E	0	91.11±2.52	38.46±2.25	3.67±0.15	2.48±0.11	14.53±0.24	1.19±0.07
	2	87.78±3.06	33.54±5.40	3.82±0.23	2.89±0.07^**	15.39±0.29^*	1.32±0.05^**
	4	85.56±4.16	32.38±5.63	3.21±0.19^**	2.34±0.21^*	12.33±0.23^**	0.92±0.05^**
	8	82.221±1.15	29.62±1.87^*	2.12±0.09^**	1.35±0.22^**	9.42±0.22^**	0.80±0.06^**
weisiensin B	0	91.11±2.52	38.46±2.25	3.67±0.15	2.48±0.11	14.53±0.24	1.19±0.07
	2	88.89±4.16	34.08±4.87	3.39±0.16^**	2.45±0.13	13.48±1.11^*	1.07±0.05^**
	4	83.33±2.65	31.11±3.06^*	3.29±0.18^**	2.30±0.21^*	13.05±0.32^*	1.00±0.15^**
	8	85.56±1.53	32.41±1.30^*	2.82±0.22^**	1.79±0.38^**	12.07±0.26^**	0.94±0.08^**

受试植物 Tested plant	化合物种类 Compounds types	浓度 Concentration /（μmol•L^-1）	化感效应指数RI值 Allelopathic response index
受试植物 Tested plant	化合物种类 Compounds types	浓度 Concentration /（μmol•L^-1）	萌发率 Germination rate	萌发指数 Germination index	根长 Root length	苗长 Seedling height	鲜质量 Fresh weight	干质量 Dry weight
黑麦草 L. perenne L.	wangzaozin A	5	-0.05	-0.15	-0.15	-0.06	-0.03	-0.22
		10	-0.05	-0.18	-0.40	-0.29	-0.37	-0.31
		25	-0.09	-0.24	-0.66	-0.46	-0.62	-0.47
	leukamenin E	5	0.01	0.02	-0.09	-0.04	-0.05	-0.03
		10	-0.05	-0.16	-0.17	-0.28	-0.17	-0.08
		25	-0.09	-0.22	-0.64	-0.40	-0.39	-0.25
	weisiensin B	5	-0.02	-0.13	-0.08	-0.10	-0.03	-0.01
		10	-0.07	-0.19	-0.18	-0.22	-0.09	-0.12
		25	-0.06	-0.18	-0.63	-0.51	-0.33	-0.25
莴苣 L. sativa L.	wangzaozin A	2	-0.05	-0.16	0.03	0.09	-0.09	0.06
		4	-0.06	-0.19	-0.19	-0.14	-0.17	-0.11
		8	-0.12	-0.30	-0.68	-0.60	-0.42	-0.19
	leukamenin E	2	-0.04	-0.13	0.04	0.14	0.06	0.10
		4	-0.06	-0.16	-0.13	-0.06	-0.15	-0.23
		8	-0.10	-0.23	-0.42	-0.46	-0.35	-0.33
	weisiensin B	2	-0.02	-0.11	-0.08	-0.01	-0.07	-0.10
		4	-0.09	-0.19	-0.10	-0.07	-0.10	-0.16
		8	-0.06	-0.16	-0.23	-0.31	-0.17	-0.21

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