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Bulletin of Botanical Research ›› 2021, Vol. 41 ›› Issue (1): 79-88.doi: 10.7525/j.issn.1673-5102.2021.01.011

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Tissue Culture via Seed Embryo for Michelia macclurei

Ying LIU   

  1. Research Institute of Tropical Forestry,Chinese Academy of Forestry,Guangzhou 510520
  • Received:2020-01-15 Online:2021-01-20 Published:2021-01-05
  • About author:LIU Ying(1963—),female,senior engineer,mainly engaged in tree biotechnology.
  • Supported by:
    The National Nonpro?t Institute Research Grant of Chinese Academy of Forestry,China(CAFYBB2017MB10)


In order to explore tissue culture and micro-propagation technical system of Michelia macclurei, the seeds from its elite families were used to carry out experimental studies at aspects of explants selection and disinfection, basic medium, PVP concentration, type and concentration of rooting promoter. The results showed that:①The embryos were well sterilized with 0.1% mercury solution for 6-9 min, and the aseptic germination rate could reach 60%-65%; ②LY medium was the best basic medium, and suitable for the proliferation and growth of most embryo lines; ③Application of PVP with concentration of 4-8 g·L-1 could significantly reduce browning degree of medium and promote growth of the shoots; ④There were significant differences in rooting ability and rooting rate among tested embryo lines, the optimal concentration of both rooting promoters was 8.5 mg·L-1, and the rooting induction effect of ABT2 root powder was significantly better than that of IBA(P<0.01); ⑤The survival rate reached above 90% for rooting plantlets in 30 d, and more than 80% for plantlets with no root in 60 d after transplanted. This study provides a theoretical and technical basis for study on tissue culture and micro-propagation of M.macclurei clones and their industrial production.

Key words: Michelia macclurei Dandy, embryo clone, PVP, basic culture media, browning

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