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Bulletin of Botanical Research ›› 2014, Vol. 34 ›› Issue (3): 393-402.doi: 10.7525/j.issn.1673-5102.2014.03.017

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EST-SSRs Locus Information Analysis from Fraxinus mandshurica Rupr.

SUN Hong-Ran;QI Feng-Hui;ZHAN Ya-Guang*;YE Peng   

  1. 1.School of Life Sciences,Northeast Forestry University,Harbin 150040;2.School of Forestry,Northeast Forestry University,Harbin 150040
  • Received:1900-01-01 Revised:1900-01-01 Online:2014-05-20 Published:2014-05-20
  • Contact: ZHAN Ya-Guang
  • Supported by:

Abstract: In order to apply the EST-SSRs technique in molecular marker aided breeding, gene localization, and gender identification of Fraxinus mandshurica Rupr., a total of 12 100 ESTs from NCBI database were analyzed. The sizes of non redundant ESTs added up to 3.02×106 bp, which can be clustered into 5 423 clads. A total of 9 489 SSRs loci (6-46 bp in length) were found via SSRIT software. Dinucleotide repeats is a predominated feature of these loci (accounting 72.86%), followed by trinucleotide repeats (24.96%) and tetranucleotide repeats (1.37%). The pattern (AG/CT)n is the most frequent one among all repeat patterns, followed by (AC/GT)n and (AT/AT)n. We designed 147 pairs of EST-SSRs primers by Primer 5.0 software. EST-SSRs in F.mandshurica Rupr displayed a relatively high level of occurrence frequency with abundant patterns. These results will pave the way for application of EST-SSRs molecular marker in F.mandshurica breeding.

Key words: Fraxinus mandshurica Rupr., simple sequence repeat, EST-SSR

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