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Bulletin of Botanical Research ›› 2014, Vol. 34 ›› Issue (1): 62-67.doi: 10.7525/j.issn.1673-5102.2014.01.009

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Cloning and Loss Expression Analysis of 5′ Flanking Sequence of UDP-glucose Pyrophosphorylase from Sugarcane

YE Bing-Ying;WANG Ting;HE Wen-Jin;QIU Si;CHEN You-Qiang;*   

  1. 1.College of Life Science,Fujian Normal University,Fuzhou　350108；2.Key Lab of Fujian Sugarcane Biology and Genetic Breeding,Ministry of Agriculture,Fuzhou　350108；3.State Key Laboratory of Developmental Biology and Neurobiology,Institution for Higher Learning in Fujian,Fuzhou　350108
  • Received:1900-01-01 Revised:1900-01-01 Online:2014-01-20 Published:2014-01-20
  • Contact: CHEN You-
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Abstract: The different lengths of 5′ flanking sequence of UGPase were cloned by Adaptor-ligation PCR. These different lengths of 5′ flanking sequences of UGPase gene were fused with the coding sequence of GUS (β-glucuronidase) gene to construct fusion genes. All the fusion genes were injected into leaves of Nicotiana tabacum for transient GUS expression. The results showed that the 5′ flanking sequence of UGPase gene did not have any promoter activity.

Key words: 5&prime, flanking sequences;loss expression;Sugarcane;UGPase

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