关键词: TCTP基因, 白粉菌, 表达半定量分析, 抗白粉病栽培小麦

Abstract: The translationally controlled tumor protein (TCTP) was initially designated as a find in mouse tumor cells. It is abundant in plant and animal cells and has many different functions. In this study, a TCTP gene was isolated as an Erysiphe graminis inducible cDNA cloned from the leaves of cultivar wheat Brock infected by E.graminis with RT-PCR and RACE technique. It encoded a polypeptide of 168 amino acids. After running a ScanProsite research, two specific TCTP signatures, named TCTP1 and TCTP2 and 7 conservation function sites were found. The studies on the expression of WbTCTP after infected by powdery mildew of race No.15 were carried out using sei-quantitative PCR technique. The expression level of WbTCTP gradually increased during the E.graminis infection. The results would provide a new way for the area of studying wheat powdery mildew resistance.

Key words: translationally controlled tumor protein gene, Erysiphe graminis, semiquantitative PCR analysis, powdery mildew resistant cultivar wheat Brock