关键词: 小麦, 几丁质酶基因, Ri质粒, 转基因植株

Abstract: Mediated by Agrobacterium rhizogene which contains plasmid pMLH7133-Chi, the genetic transformation experiment was carried out with the callus of Spring Wheat Dongnong 7742, Longmai 9814, and Hite as the acceptor in the way of orthogonal test of L₉(3⁴). The result indicates that the best group is Dongnong 7742, 100 μm of Acetosyringone, A. rhizogene’s OD₆₀₀=0.8 and about two days’ co-cultivation; the order of influential factors is: genotype>concentration of Acetosyringone>concentration of A. rhizogene> co-culture period. 138 regenerated plants were obtained in the experiment. Then detected by PCR amplification and PCR-Southern, the result suggests that the foreign Chitinase genes have already been introduced into 5 wheat genomes of all. Three of the five plants were detected again by RT-PCR and Chitinase activity methods, which proved that the foreign Chitinase gene has expressed steadily in wheat genome.

Key words: wheat, Chitinase gene, Ri plasmid, transgenic plant