甘蔗SPSⅢ5′侧翼序列缺失表达载体的构建及其转化烟草的研究

doi:10.7525/j.issn.1673-5102.2011.06.010

植物研究 ›› 2011, Vol. 31 ›› Issue (6): 696-701.doi: 10.7525/j.issn.1673-5102.2011.06.010

甘蔗SPSⅢ5′侧翼序列缺失表达载体的构建及其转化烟草的研究

池朝华^1,2；陈玲^1,2；周平³；高玉娜^1,2；叶冰莹^1,2；陈由强^1,2*

1.福建师范大学生命科学学院，福州　350108；2.农业部甘蔗遗传改良重点开放实验室，福州　350108；3.福建省农业科学院果树研究所，福州　350013

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-11-20 发布日期:2011-11-20
通讯作者: 陈由强
基金资助:

Construction of SPS Ⅲ in Sugarcane 5′ Flank Sequence Deletion Expression Vectors and the Transformation in Tobacco

CHI Zhao-Hua;CHEN Ling;ZHOU Ping;GAO Yu-Na;YE Bing-Ying;CHEN You-Qiang;*

1.College of Life Sciences,Fujian Normal University,Fuzhou　350108；2.Key Laboratory of Genetics Improvement for Sugarcane,Ministry of Agriculture,Fuzhou　350108；3.Fruit Research institute,Fujian Academy of Agricultural Sciences,Fuzhou　350013

Received:1900-01-01 Revised:1900-01-01 Online:2011-11-20 Published:2011-11-20
Contact: CHEN You-Qiang
Supported by:

摘要/Abstract

摘要： 蔗糖磷酸合成酶是糖代谢调节的关键酶之一，根据本实验室克隆到的SPSⅢ5′侧翼序列,构建了3个侧翼序列缺失表达载体，转化云烟85成功获得转基因植株，可用于核心启动子确定的研究。转基因植株叶片冰冻切片结果表明3段缺失侧翼序列均有启动子活性。

关键词: 蔗糖磷酸合成酶Ⅲ, 核心启动子, 绿色荧光蛋白

Abstract: Sucrose phosphate synthase is one of the key enzymes in the metabolism of sucrose. Based on the 5′ flank sequence of SPS Ⅲ cloned in our lab, 3 flank sequence deletion expression vectors were constructed and transformed in YUNYAN 85 tobacco leaves. Three transgenic plants were obtained, which can be used in the identification of core promoter. The results revealed that all these three deletion flank sequences have promoter activity.

Key words: sucrose phosphate synthase, core promoter, green fluorescent protein

中图分类号:

S566.1

池朝华;陈玲;周平;高玉娜;叶冰莹;陈由强;*. 甘蔗SPSⅢ5′侧翼序列缺失表达载体的构建及其转化烟草的研究[J]. 植物研究, 2011, 31(6): 696-701.

CHI Zhao-Hua;CHEN Ling;ZHOU Ping;GAO Yu-Na;YE Bing-Ying;CHEN You-Qiang;*. Construction of SPS Ⅲ in Sugarcane 5′ Flank Sequence Deletion Expression Vectors and the Transformation in Tobacco[J]. Bulletin of Botanical Research, 2011, 31(6): 696-701.

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甘蔗SPSⅢ5′侧翼序列缺失表达载体的构建及其转化烟草的研究

Construction of SPS Ⅲ in Sugarcane 5′ Flank Sequence Deletion Expression Vectors and the Transformation in Tobacco

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