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植物研究 ›› 2007, Vol. 27 ›› Issue (2): 224-228.doi: 10.7525/j.issn.1673-5102.2007.02.021

• 论文 • 上一篇    下一篇

花楸腋芽增殖途径快繁技术研究

郭艳茹1;詹亚光2*;纪丽丽3   

  1. 1.运城学院生命科学系,运城 0440002.东北林业大学生命科学学院,哈尔滨 150040 3.黑龙江林业职业技术学院,牡丹江 157011
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-03-20 发布日期:2007-03-20
  • 通讯作者: 詹亚光
  • 基金资助:
     

The Rapid Propagation Technology of Sorbus pohuashanensis by Axillary Bud Regeneration Way

GUO Yan-Ru;ZHAN Ya-Guang*;JI Li-Li   

  1. 1.Department of Life Science,Yuncheng University,Yuncheng 044000 2.College of Life Science, Northeast Forestry University,Harbin 1500403.Heilongjiang Forestry Vocation-Technical College,Mudanjiang 157011
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-03-20 Published:2007-03-20
  • Contact: ZHAN Ya-Guang
  • Supported by:
     

摘要: 选用花楸茎节为外植体进行组培腋芽增殖途径研究,结果表明:利于花楸生长的最适宜基本培养基为MS培养基;芽诱导培养基为1/2MS+6-BA 2.5 mg·L-1+2.4-D 0.5 mg·L-1;增殖培养基为MS+6-BA(1.600~2.100)mg·L-1+NAA(0.140~0.230) mg·L-1,平均繁殖系数达到13倍以上;继代壮苗培养的最佳培养基为MS+6-BA 0.2 mg·L-1+IBA 0.2 mg·L-1;在1/2MS+IBA 0.3 mg·L-1培养基上,平均生根数为5.96,生根率达90.20%。将生根后的组培苗移栽至腐殖土:泥炭土:河沙(比例为3:2:1)的基质中,成活率达95.55%。

关键词: 花楸腋芽, 组织培养, 快速繁殖

Abstract: In order to study the rapid propagation technology for Sorbus pohuashanensis stem segments with axillary buds were chosen as explants. The results showed that the best basic media was MS; axillary buds were induced successfully on MS basal media supplemented with 6-BA(2.5 mg·L-1) and 2.4-D(0.5 mg·L-1); the optimum medium for bud proliferation was MS+6-BA(1 600~2 100)mg·L-1+NAA(0.140~0.230)mg·L-1, 13 shoots per explant could be obtained at average;the shoots grew strongly on MS+6-BA0.2 mg·L-1+IBA0.2 mg·L-1; And 1/2MS+IBA0.3 mg·L-1 was the best medium for shoot rooting,with the rooting rate at 90.20%,and the average root number of 5.96;Plantlets survival rate reached 95.55% when transplanted on the mixture(humus 3:peat soil 2:sand 1).

Key words: Sorbus pohuashanensis axillary bud, tissue culture, rapid propagation

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