马铃薯杂种F1无性株系的ISSR鉴定

doi:10.7525/j.issn.1673-5102.2013.02.021

植物研究 ›› 2013, Vol. 33 ›› Issue (2): 243-247.doi: 10.7525/j.issn.1673-5102.2013.02.021

马铃薯杂种F₁无性株系的ISSR鉴定

甘霖¹；于肖夏²；鞠天华³；于卓^1*；蒙美莲¹；张自强¹；李长青¹

1.内蒙古农业大学农学院，呼和浩特　010019；2.中国农业大学农业部作物遗传改良重点实验室，北京　100193；3.赤峰天泽生物科技有限公司，赤峰　025457

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2013-03-20 发布日期:2013-03-20
通讯作者: 于卓
基金资助:

ISSR Identification of Clone Plants of Solanum tuberosum Hybrids F₁

GAN Lin;YU Xiao-Xia;JU Tian-Hua;YU Zhuo*;MENG Mei-Lian;ZHANG Zi-Qiang;LI Chang-Qing

1.College of Agronomy,Inner Mongolia Agricultural University,Hohhot　010019；2.Key Laboratory of Crop Genomics and Genetic Improvement,Ministry of Agriculture,China Agricultural University,Beijing　100193；3.Chifeng Tianze BioTechnology CO,LTD,Chifeng　025457

Received:1900-01-01 Revised:1900-01-01 Online:2013-03-20 Published:2013-03-20
Contact: YU Zhuo
Supported by:

摘要/Abstract

摘要： 为给马铃薯新品种选育提供可靠材料，采用ISSR分子标记对2个马铃薯杂交组合‘J07-4’×‘陇薯6号’和‘J07-6’×‘陇薯6号’杂交种F₁无性繁殖株系的真实性进行了鉴定。结果从152个ISSR引物中筛选出适于‘J07-4’×‘陇薯6号’杂种F₁ 5个无性株系鉴定的3个ISSR引物AF18550、AW75511和AW20617及‘J076’×‘陇薯6号’杂种F₁ 7个无性株系鉴定的2个ISSR引物AW20607和AF18549；以子代中含有父本特征带为主要依据，鉴定出杂种F₁共12个无性繁殖株系均为真实的杂交种，并建立了杂种F₁不同无性株系间的ISSR指纹图。表明ISSR分子标记技术用于马铃薯杂种F₁无性株系鉴定是可行的。

关键词: 马铃薯, 杂种F₁, 无性株系, ISSR鉴定

Abstract: In order to provide reliable materials for potato varieties breeding, the study was to identify the authenticity of clones of hybrids F₁ of two potato combinations, ‘J07-4’×‘Longshu No.6’ and ‘J07-6’×‘Longshu No.6’, using ISSR molecular markers. The results were that five primers were selected from 152 ISSR primers, primers AF18550, AW75511 and AW20617 were suitable to identify 5 clones of ‘J07-4’×‘Longshu No.6’ hybrids F₁, and AW20607 and AF18549 were suitable to identify 7 clones of ‘J07-6’×‘Longshu No.6’ hybrids F₁. A total of 12 clones were identified as true hybrids based on the paternal-specific bands in progeny’s bands. ISSR fingerprints of different clones of hybrids F₁ were established. It showed that it was feasible to use ISSR molecular markers to identify clones of potato hybrids F₁.

Key words: Potato, hybrids F₁, clones, ISSR identification

中图分类号:

S532
S336

甘霖;于肖夏;鞠天华;于卓*;蒙美莲;张自强;李长青. 马铃薯杂种F₁无性株系的ISSR鉴定[J]. 植物研究, 2013, 33(2): 243-247.

GAN Lin;YU Xiao-Xia;JU Tian-Hua;YU Zhuo*;MENG Mei-Lian;ZHANG Zi-Qiang;LI Chang-Qing. ISSR Identification of Clone Plants of Solanum tuberosum Hybrids F₁[J]. Bulletin of Botanical Research, 2013, 33(2): 243-247.

[1]	李登高, 林睿, 穆青慧, 周娜, 张焱如, 白薇. 马铃薯StCRKs基因家族的鉴定分析及响应逆境信号的表达[J]. 植物研究, 2022, 42(6): 1033-1043.
[2]	李登高, 林睿, 穆青慧, 周娜, 张焱如, 白薇. 马铃薯StNPR4基因的克隆与功能分析[J]. 植物研究, 2022, 42(5): 821-829.
[3]	白江平;;崔同霞;;张金文;;张俊莲;;王蒂;*. 马铃薯块SGAs合成途径关键基因表达量的GGE双标图分析[J]. 植物研究, 2014, 34(2): 238-244.
[4]	范阿棋;;毛娟;;刘思妍;;张俊莲;;王蒂;;白江平;. 马铃薯StSnRK*2.2基因克隆与生物信息学分析[J]. 植物研究, 2013, 33(3): 294-301.

马铃薯杂种F₁无性株系的ISSR鉴定

ISSR Identification of Clone Plants of Solanum tuberosum Hybrids F₁

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 4

编辑推荐

Metrics

本文评价